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Western Lightning ONE Femto, Chemiluminescent Substrate (500 mL)

A versatile, mid-level pre-mixed, one-component chemiluminescent horseradish peroxidase (HRP) substrate appropriate for a wide range of antibody dilutions, Western Lightning ONE Femto can detect mid-femtogram protein levels in western blots with a high signal intensity.

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Unit Size
List Price
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NEL140001EA
25 mL
73.00 USD
 
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NEL141001EA
250 mL
259.00 USD
 
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NEL142001EA
2 x 250 mL
409.00 USD
 
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Overview

Get accurate detection of both low and high abundance proteins in the same experient. Part of our premier line of premixed chemiluminescnet HRP substrates, Western Lightning ONE Femto offers the flexibility of mid-femtogram detection with excellent dynamic range. This substrate offers sensitive and consistent detection of proteins with no mixing or measuring parts required. Stored and ready-to-use at room temperature, the 12 month extended shelf life means you can buy and use Western Lightning ONE Femto with confidence. Unit size 2 x 250 mL.

Comparison of Western Lightning ONE Versus Major Competitors

Performance and Signal Duration

  • Western blotting detection of HDAC-1 in HeLa cell lysate
  • Exposure time is 120 secs for each time point
  • Images captured with ImageQuant LAS 4000 from GE Healthcare
Performance Signal Duration
Detection Level: Mid-picogram
pico pico
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:2000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:20000
Detection Level: Mid-femtogram
femto femto
  • Two-fold dilution series of HeLa whole cell lysate from 5 μg to 0.078 μg of total protein on nitrocellulose membrane
  • Primary antibody: Rabbit-anti Human HDAC-1 1:5000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:75000
Detection Level: Low-femtogram
femto ultra femto ultra
  • Two-fold dilution series of HeLa whole cell lysate from 2.5 μg to 0.039 μg of total protein on nitrocellulose membrane
  • Primary antibody:  Rabbit-anti Human HDAC-1 1:10000
  • Secondary antibody: Goat anti-rabbit IgG HRP (2mg/mL) 1:200000

Specifications

Detection Method Chemiluminescence
Product Brand Name Western Lightning ONE
Sample Type Chemiluminescent Substrate
Shipping Condition Ambient
Unit Size 2 x 250 mL
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Application Note

Western Blot Validation of CRISPR-Cas9-induced Functional Knockout using Synthetic sgRNA Molecules

Any well-designed CRISPR experiment must include a method to assess the accuracy and reliability of predicted gene editing results. This application note demonstrates how Horizon Discovery’s Edit-R predesigned synthetic sgRNAs result in high efficacy knockout o ...

PDF 1 MB

Flyer

The Power of Western Lightning ONE HRP Chemiluminescent Substrates

One-Reagent Substrates for Western Blotting

Get the consistency you demand from your western blot experiments with pre-mixed, one-reagent horseradish peroxidase (HRP) chemiluminescent substrates.

Western Lightning ONE, PerkinElmer’s premier line of premixed one-component chemilumines ...

PDF 1002 KB