Label antibodies, small molecules, proteins, or peptides with VivoTag® NIR fluorescent dyes.
Available as either NHS esters or maleimide reactive dyes, making conjugation to either free amine (-NH3) or free thiol (-SH) containing molecules possible, our VivoTag fluorescent dyes have been validated for in vivo and in vitro applications. Please refer to the configuration table below to see which VivoTag fluorescent agent is best for your research studies.
VivoTag is available in multiple sizes and wavelengths to fit your imaging needs.
true falseFor laboratory use only. This product is intended for animal research only and not for use in humans.
VivoTag® 645 Fluorochrome is an amine-reactive near infrared (NIR) fluorochrome for coupling via a NHS ester linkage to peptides, small molecules, proteins, antibodies or macromolecules. Quantity = 5 mg.
| Product | Abs/Em (nm) | |
| VIVOTAG | ||
| VivoTag 645 | 643/660 | Suitable for labeling free amines (typically lysine residues) |
| VivoTag 680 XL | 665/688 | |
| VivoTag 800 | 785/810 | |
| VIVOTAG MAL | ||
| VivoTag 645 MAL | 643/660 | Suitable for labeling free thiols (typically cycteine residues) |
| VivoTag 680 XL MAL | 665/688 | |
| VIVOTAG-S | ||
| VivoTag 680-S | 665/688 | "S" for self-quenching. When a protein is labeled with multple VivoTag-S molecules, there is a shift in the absorbance of the dye, which will reduce the signal. Only choose 'S' form if your labeling ratio of dye:protien will be 1:1. If multiple dyes are to be conjucated onto your protein, the non-"S" version should be used. Suitable for labeling free amines (typically lysine residues) |
| VivoTag 750-S | 750/775 | |
| Optical Imaging Classification | Fluorescence Imaging |
|---|---|
| Product Brand Name | VivoTag |
| Quantity in a Package Amount | 1.0 Units |
| Shipping Condition | Blue Ice |
| Unit Size | 5 mg |
| Wave Length | 645 nm |
VivoTag® 645 is a red fluorescent labeling agent containing an N-hydroxysuccinimide (NHS)-ester. Labeling reagents, such as VivoTag 645 reagent, are commonly used for labeling proteins. The NHS ester moiety on VivoTag 645 reacts with amino groups to form amide bonds. Lysines within proteins, including antibodies, are available as targets for this chemical conjugation.,Protein labeling efficiency may vary depending upon the type of protein labeled, so different conjugation ratios may need to be attempted to attain success. For red fluorophore conjugation, ratios of fluorophore to protein of 4:1 have been seen to generate effective imaging agents. Not all antibodies or proteins (independent of target specificity) make good imaging agents, due to long half-lives and/or excessive accumulation in non-target sites.
Complete suite of in vivo fluorescence tomography imaging solutions from PerkinElmer lets you discover more about biological targets, processes and pathways, directly in the living animal.
Researchers trust our in vivo imaging solutions to give them reliable, calibrated data that reveals pathway characterization and therapeutic efficacies for a broad range of indications. Our reagents, instruments, and applications support have helped hundreds of research projects over the years. And our hard-earned expertise makes us a trusted provider of pre-clinical imaging solutions— with more than 9,000 peer reviewed articles as proof.
VivoTag® 645 is an amine reactive red fluorochrome (NHS ester) for molecular labeling applications.VivoTag 645 enables optimized and integrated imaging applications in both in vivo and in vitro applications. VivoTag 645’s superior brightness makes it particularly well suited for the labeling and imaging of proteins, antibodies, and small moleculesin a wide range of research areas.
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