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VICTOR Nivo Multimode Microplate Reader

Now with Alpha Technology, the VICTOR® Nivo system packs all popular detection modes into the industry’s smallest multimode reader footprint. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all standard technologies (top only reading for Alpha). Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser environments. Combine with our assays and reagents to get you started right away.

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Part Number HH35000500
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For research use only. Not for use in diagnostic procedures.


The VICTOR Nivo system is a high-performance filter-based multimode microplate reader equipped with all major detection technologies:

  • Absorbance
  • Luminescence
  • Fluorescence intensity
  • Time-resolved fluorescence
  • Fluorescence polarization
  • Alpha

It’s a compact, light-weight multimode plate reader designed for life science research laboratories performing routine low-throughput assays, or assay development work, with diverse application requirements. When combined with the stacker, throughput for the VICTOR Nivo is increased and productivity is enhanced by enabling users to set up walk away automation so they can focus on their research and not on managing plates.

The VICTOR Nivo system’s software has a modern, workflow-oriented user interface which is easy to learn and use and includes pre-written application protocols to get users productive quickly. In addition, it allows you to use your test results to perform basic data analysis immediately after running your assay. For complex calculations, optional MyAssays® Desktop Pro analysis software is available for use in the lab or at your desk. With our Enhanced Security software option, it contains technological tools to help guide you through the complexity of compliance for 21 CFR Part 11.

The browser-based control software is independent of operating system, so it can be controlled via a variety of touchscreen devices, for example Windows or Mac OS PCs or tablets.

The VICTOR Nivo microplate reader can be operated via WiFi or a network connection, enabling experiments to be set-up, run and monitored remotely, such as from outside a biosafety cabinet or from a neighbouring lab.

Dynamic Rainbow Filter Exchanger

Every VICTOR Nivo comes rainbow-ready with our dynamic double-layered filter wheel system that can store up to 32 filters. When fully loaded, the system is equipped to address virtually any application, with better sensitivity than a monochromator.

Filters are:

  • Exchanged between the inner and outer wheels
  • Used for either excitation or emission light path
  • Locked in position so they don't get lost - ideal for multiuser labs

The Spectra Viewer tool helps you visualize the spectral compatibility of fluorophores and fluorescent labelled probes so you can determine what filters are best for detection. Choose the appropriate PerkinElmer multimode plate reader from the Select Machine drop-down menu. After selection, filters for the chosen instrument will be displayed automatically.

See full list of filters in the Resources tab.

Detection Technologies

For absorbance measurements using the VICTOR Nivo multimode plate reader, there is a choice of either a filter- or a spectrometer-based system. With the spectrometer-based plate reader, full spectrum absorbance measurements are ultra-fast – 230 to 1000 nm at selectable resolutions (2.0 nm, 5.0 nm, 10 nm) in less than 1 second per well.

Up to eight discrete wavelengths can be detected at once in a single measurement with no wavelength switching, and the spectrometer-based VICTOR Nivo system also allows the detection of a wide range of dyes or measurement of samples with unknown absorbance spectra.

Features & Benefits

  • Compact, lightweight multimode plate reader which frees-up bench space and is easy to move into a biosafety cabinet or another lab
  • Internal dynamic filter wheel system, with space for up to 32 filters, provides ready access to filters for a large number of dyes
  • For absorbance, choice of filter-based detection for best sensitivity or spectrometer for wavelength flexibility
  • Time resolved fluorescence certified for use with HTRF® technology
  • Top and bottom reading of all standard technologies (absorbance, luminescence, fluorescence intensity, time-resolved fluorescence and fluorescence polarization), top reading only for Alpha; with high performance for plate formats from 1 to 1536-wells
  • Stacker option for batch processing of up to 20 microplates
  • Enhanced Security software for regulated environments provides technological controls and features that support 21 CFR Part 11 compliance
  • Optional dispenser for applications such as fast kinetics, flash luminescence assays, or dual addition assays
  • Integrated temperature control (up to 65 °C) and optional gas control unit to keep cells viable during long term kinetic assays
  • Browser-based software enables the plate reader to be controlled from a variety of devices – PC, laptop or tablet
  • Can be controlled via network or WiFi to facilitate remote working and data access

Selected Publications:

  • Euxanthone inhibits traumatic spinal cord injury via anti-oxidative stress and suppression of p38 and PI3K/Akt signaling pathway in a rat model. Yao, R., Ren, L., Wang, S., Zhang, M., & Yang, K. (2021). Translational neuroscience, 12(1), 114–126.
  • N-terminal domain antigenic mapping reveals a site of vulnerability for SARS-CoV-2. McCallum, M., De Marco, A., Lempp, F. A., et al. (2021). Cell, 184(9), 2332–2347.e16. Advance online publication.
  • A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting. Sarah M Hicks, Kai Pohl, Teresa Neeman, et al. The Journal of Infectious Diseases, Volume 223, Issue 1, 1 January 2021, Pages 10–14.
  • Berberine-Loaded Thiolated Pluronic F127 Polymeric Micelles for Improving Skin Permeation and Retention. Niu J, Yuan M, Chen C, et al. Int J Nanomedicine. 2020;15:9987-10005. Published 2020 Dec 8.
  • Phylogenetic analysis of the first four SARS-CoV-2 cases in Chile. Castillo AE, Parra B, Tapia P, et al. J Med Virol. 2020;92:1562–1566.
  • Development of Novel Diclofenac Analogs Designed to Avoid Metabolic Activation and Hepatocyte Toxicity. Yasuhiro Tateishi, Tomoyuki Ohe, Mai Ogawa, Kyoko Takahashi, Shigeo Nakamura, and Tadahiko Mashino. ACS Omega 2020 5 (50), 32608-32616.
  • Enzymatically synthesized 2'-fluoro-modified Dicer-substrate siRNA swarms against herpes simplex virus demonstrate enhanced antiviral efficacy and low cytotoxicity. Levanova, A. A., Kalke, K. M., Lund, L. M., et al. (2020). Antiviral research, 182, 104916.
  • SARS-CoV-2 Infection Dysregulates the Metabolomic and Lipidomic Profiles of Serum. Chiara Bruzzone, Maider Bizkarguenaga, Rubén Gil-Redondo, et al. iScience, Volume 23, Issue 10, 101645, October 23, 2020.
  • The ABCG2 multidrug transporter is a pump gated by a valve and an extracellular lid. Khunweeraphong, N., Szöllősi, D., Stockner, T., & Kuchler, K. (2019). Nature communications, 10(1), 5433.
  • Structural basis for RNA translocation by DEAH-box ATPases. Hamann, Florian et al. Nucleic acids research vol. 47,8 (2019): 4349-4362.

For additional publications, please visit Google Scholar.


Depth 50.0 cm
Detection Method Absorbance, Time-Resolved Fluorescence (TRF), Luminescence, Alpha, Fluorescence Intensity, Fluorescence Polarization
Height 27.0 cm
Portable Yes
Product Brand Name VICTOR Nivo
Weight 13.0 kg
Width 20.0 cm


Modalities VICTOR Nivo F VICTOR Nivo S VICTOR Nivo Alpha F VICTOR Nivo Alpha S Standard Features Optional Extras
(Filter-based (F) or Spectrometer (S))
  • Top and bottom detection of all technologies (except Alpha) for plate formats up to 1536-wells
  • Temperature control up to 65°C
  • Shaker with three modes (linear, orbital, and double orbital)
  • Browser-based control software
  • Wi-Fi modem for remote control
  • Basic data analysis software
  • Dispenser (dual injectors)
  • Gas control (CO2 & O2) Automation Control SDK
  • 15-in. laptop
  • MyAssays Desktop Pro analysis software
  • Stacker
  • Enhanced Security software supporting 21 CFR Part 11 compliance in regulated environments
  • Test plate for alignment and system check
  • Field upgrades from standard to advanced models (includes time-resolved fluorescence and fluorescence polarization)
Fluorescence Intensity
Time-resolved Fluorescence Optional Optional Optional Optional
Fluorescence Polarization Optional Optional Optional Optional
Resources, Events & More
  • All

Application Note

Adaptation of a Bacterial Growth Detection Assay on the VICTOR Nivo for Measurement of Antibiotic Effects

Monitoring of bacterial growth is a fundamental technique in microbiology. The most widely used method for enumerating bacteria is plate-based counting, which requires serial dilutions of the bacterial stock culture and manual counting of colonies after plating. The procedure is very time consumi ...

High-Throughput DNA Quantification for Next Generation Sequencing on the VICTOR Nivo

There are many different methods of quantification available on the market for DNA samples and each can vary in precision, accuracy and throughput. Typically, DNA in next generation sequencing (NGS) workflows is measured using tube-based fluorescent assays such as Qubit® or qPCR. H ...

Quantification of ADAMTS13 Activity by FRET Assay and Kinetic Data Analysis Using MyAssays Desktop on the VICTOR Nivo

In this application note, the VICTOR® Nivo multimode plate reader is used in combination with MyAssays® Desktop Software to perform kinetic assays and analyze data in a straightforward and efficient manner.

ADAMTS13 is a biomarker of thromboti ...

PDF 705 KB
Quantification of Proteins by UV-Vis Absorbance Using VICTOR Nivo with Microvolume Plates

Knowing the protein concentration is commonly required in studies of protein biochemistry and molecular biology. A direct method of measuring protein concentration is to determine the absorbance spectrum at 280 nm. With this UV based approach, it enables scientists to rapidly determine the concen ...

The Utility of Adeno-Associated Viruses (AAVs) in Therapeutic Approaches for Monogenic Neurological Diseases

Gene therapy is a very powerful tool that is currently being explored to combat disorders with underlying genetic causes. Within the field of neurological diseases, there is great interest looking at rare diseases of monogenic origin with the hope of developing disease-modifying gene therapies, a ...

PDF 454 KB


Complete Neuroscience Solutions for Research and Discovery

Like the brain, neuroscience has a multitude of layers. Our broad solutions, innovative technologies, and proven expertise help researchers like you understand neurological diseases so that patients can benefit from better treatments and therapies.

VICTOR Nivo Multimode Plate Reader Brochure

This Brochure describes the VICTOR Nivo Multimode Plate Reader.



Choosing the Right Plate Reader

Selecting the right plate reader for your lab can be challenging. There are multiple factors to consider including: detection type, additional features, support, and price. With numerous options for each variable, it can be confusing to navigate the selection process. Focusing on striking a balan ...

Detection Technologies for Your Scientific Breakthroughs

There are many options available today to help labs with their scientific research. Understanding the latest detection technologies, assay platforms, and microplates can inform you to make better decisions and set your lab up for success. PerkinElmer offers an optimal solution to meet your divers ...

Solving the Complexity of Compliance

Reaching compliance according to the laws and regulations of each country can be a tedious, complicated process, and being GxP compliant (FDA 21 CFR Part 11, EU Annex 11, etc.) is necessary in order to deliver therapeutic treatments to market for pharmaceutical and biotechnology companies, includ ...



Infographic - Solutions for your Toxicology Lab

Infographic Poster showing the range of different analytical solutions for Toxicology Labs; from GC and LC, to ICP-MS and UV-Vis. Benchtop and floor standing chromatography, spectroscopy and thermal solutions for everyday robust and reliable analyses.


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Product Info

Technical Note

DNA Quantification Using the VICTOR Nivo and Micro-Volume Plates

All life science related fields from basic research in academia to commercial diagnostics laboratories rely on quick and simple, yet highly accurate quantification of nucleic acids. Commonly, photometers are employed to determine quality, purity, and concentration by measuring the absorption of the ...

PDF 495 KB
High-Performance Alpha Technology on VICTOR Nivo

Alpha technology is a homogenous bead-based proximity assay with a solution for just about every research application. To bring this technology to research environments with lower budget capabilities, the VICTOR® Nivo multimode plate reader is now available with a ...

PDF 959 KB
Speed Up Your Immunoassays with AlphaLISA Detection on VICTOR Nivo

Using ELISA technology for immunoassays on a plate reader is laborious and time-consuming because it requires numerous wash steps. In the field of drug discovery, technologies that give robust, sensitive, automatable, and reproducible immunoassays at a lower cost are highly desirable.

Alpha ...

PDF 975 KB
Using the VICTOR Nivo for Cell-Based HTRF Assays

Cell-based assays are routinely employed in R&D to reveal compound induced effects in vitro. In this technical note, we describe the usage of the VICTOR® Nivo multimode plate reader for alpha-tubulin acetyl-K40 detection in a cellular assay based on HTRF


White Paper

Advancing Neurological Treatments at Synaptic Speed

There is a huge unmet need for the development of treatments that target the underlying causes of neurodegenerative diseases. To address the high failure rates and low investment returns of traditional neuroscience R&D approaches, companies are increasingly adopting alternative R&D business model ...

PDF 507 KB
Guidance for Ensuring Your VICTOR Nivo Multimode Plate Reader Complies with 21 CFR Part 11

Ensuring that laboratory processes are compliant with the regulations of the U.S. Food and Drug Administration (FDA), such as 21 CFR Part 11, and other references, e.g. Annex 11 published by the European Commission, can be time-consuming and laborious, requiring meticulous documentation of proced ...

Leveraging the Microbiome to Strengthen Immune Therapeutic Response

Researchers are striving to determine where and when relevant bacteria play a role in cancer progression and whether those interactions are localized to the gastrointestinal tract or occur in other tissues before malignancy. Understanding the role bacteria play, whether it be oncogenesis, tumor p ...

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