PerkinElmer
check quantity

LANCE Ultra ULight™-CREBtide, 0.125 nmole

Generic peptide substrate for use in TR-FRET biochemical kinase assays. Refer to Selector Guide for validated kinases. CREBtide peptide (sequence: CKRREILSRRPSYRK) labeled with ULight dye. Synthetic peptide derived from human cAMP Response Element Binding (CREB) Protein (phosphorylation site: Ser133). CREB (Ser 133) can be phosphorylated by a variety of kinases including MSK1, PKA and PKC isoforms.

You successfully added item(s) to your cart

Part Number
Unit Size
List Price
Your Price
Quantity
TRF0107-C
0.125 nmol
313.00 USD
more
TRF0107-D
0.5 nmol
352.00 USD
more
TRF0107-M
5 nmol
900.00 USD
more
Buy Now

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Detail Information

LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:

  • Test conditions: Rapid assay setup to move straight into optimized assay performance
  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing
  • Phospho-motifs: Substrate sequences are provided
  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For ULight substrates:

  • 0.5 nmoles = 1,000 assay points
  • 5 nmoles = 10,000 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.

Specifications

Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore ULight
Molecular Modification Phosphorylation
Product Brand Name LANCE Ultra
Shipping Condition Dry Ice
Unit Size 0.125 nmol
Resources, Events & More
  • All

Brochure

Guide

LANCE® Ultra Kinase Assay Selection Guide

LANCE® Ultra TR-FRET reagents comprise the widest portfolio of validated kinase assay offerings available for rapid, sensitive ,and robust screening of purified kinase targets in a biochemical format.

PDF 1 MB