TSA® Plus DNP (HRP) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. The deposited DNP is detected using an anti-DNP hroseradish peroxidase, with appropriate substrate.
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TSA Plus DNP System for chromogenic detection with horseradish peroxidase:
| Detection Method | Fluorescence, Chromogenic |
|---|---|
| Hapten | DNP |
| Product Brand Name | TSA Plus |
| Shipping Condition | Blue Ice |
| Unit Size | 50-150 slides |
TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.
The extraordinary sensitivity of the Tyramide Signal Amplification (TSA™) kits from PerkinElmer lets you see previously undetectable levels of protein and nucleic acid. The resolution is remarkable. And with multi-target detection, you can get more information from each experiment. It’s clear to see. TSA makes it easy to gain valuable insight from your immunohistochemistry (IHC) and immunocytochemistry (ICC) results.
Tyramide Signal Amplification (TSA™) provides remarkable sensitivity enhancement for your ISH experiments without the drawbacks associated with other methods. Signal amplification with TSA allows detection at levels as low as asingle copy and enables the use of shorter probes for more precise localization of targets. The TSA reaction occurs within 10 minutes, and labels are bonded covalently, ensuring outstanding resolution.
TSA Plus DNP technology uses HRP to catalyze the deposition of the dinitrophenyl (DNP) labeled amplification reagent onto tissue sections or cell preparation surfaces that have been previously blocked with proteins. The reaction is quick (less than 10 minutes) and results in the deposition of numerous DNP labels immediately adjacent to the immobilized HRP enzyme.
