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TSA Plus Cyanine 3.5, 50-150 Slides

TSA® Plus Cyanine 3.5 (Cy3.5) detection kit, for amplification of signal in immunohistochemistry (IHC), immunofluorescence (IF) or in situ hybridization protocols. This kit amplifies by depositing extra Cy3.5 in the localized area of the probe or antibody.

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Unit Size
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NEL763E001KT
1 Kit
216.00 USD
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NEL763001KT
50-150 slides
394.00 USD
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NEL763B001KT
250-750 slides
1727.00 USD
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Detail Information

TSA Plus kits deliver ultimate sensitivity in immunohistochemistry (IHC), immunofluorescence (IF), and in situ hybridization (ISH) assays. This widely referenced method uses horseradish peroxidase (HRP) to mediate intense covalent deposition of detection labels at very close proximity to the epitope or sequence of interest. TSA Plus has been employed for quantitative detection in a wide variety of sample types including formalin-fixed, paraffin-embedded (FFPE) tissue, cryosections and cultured cells.

 

TSA Plus Cyanine 3.5 provides another option for fluorescent detection with spectral characteristics that are generally similar to mCherry, Alexa Fluor 568® or Texas Red®. It has maximum absorbance at 581 nm and maximum emission at 596 nm.

 

TSA Plus Cyanine 3.5 is ideal for multiplexed IHC or ISH in combination with other TSA fluorophores. The Opal™ protocol allows simultaneous measurement of 6 or more biomarkers in FFPE tissue.

 

Specifications

Fluorophore Cy3.5
Product Brand Name TSA Plus
Shipping Condition Blue Ice
Unit Size 50-150 slides
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Brochure

TSA™ Signal Amplification for IHC, ICC, IF

The extraordinary sensitivity of the Tyramide Signal Amplification (TSA™) kits from PerkinElmer lets you see previously undetectable levels of protein and nucleic acid. The resolution is remarkable. And with multi-target detection, you can get more information from each experiment. It’s clear to see. TSA makes it easy to gain valuable insight from your immunohistochemistry (IHC) and immunocytochemistry (ICC) results.

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TSA™ Signal Amplification for ISH

Tyramide Signal Amplification (TSA™) provides remarkable sensitivity enhancement for your ISH experiments without the drawbacks associated with other methods. Signal amplification with TSA allows detection at levels as low as asingle copy and enables the use of shorter probes for more precise localization of targets. The TSA reaction occurs within 10 minutes, and labels are bonded covalently, ensuring outstanding resolution.

PDF 2 MB

Flyer

Opal Multiplex Tissue Staining Flyer

Opal™ is a practical workflow for specific staining of multiple tissue biomarkers able to reach to 8-plex and beyond in a single image.

PDF 2 MB