Normalization plate kit for the TopCount liquid scintillation counter. This 96-well SPC (single photon counting) plate is used for TopCount luminescence normalization (for the 2, 6, or 12 detector system). The plate itself is a LumaPlate (Yttrium silicate, solid scintillator), with 3H thymidine impregnated epoxy.
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Each time you create a new assay you must normalize the instrument’s detectors. This is called per assay normalization. You should normalize the instrument’s detectors before you use the newly created assay to count samples.
During the normalization procedure, the system calculates a factor for each detector that compensates for counting differences between detectors. When the system applies the factor to the value measured by the detector, the final result is that different detectors counting the same sample will yield similar CPM and tSIS values. This balances the responses of the detectors against one another.
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