APC-labeled streptavidin, for use in LANCE® TR-FRET protein binding assays.
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PerkinElmer offers two versions of SureLight APC-SA: one for protein binding assays, and one for kinase assays. SureLight APC-SA for protein binding assays has a ratio of APC:streptavidin that has been optimized to achieve the best performance in protein binding applications.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE SureLight|
|Shipping Condition||Blue Ice|
|Unit Size||30 mg|