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Streptavidin AlphaPlex™ 545 Acceptor Beads 5 mg

Streptavidin-coated AlphaPlex™ 545 (Terbium) Acceptor beads, for use in Alpha no-wash multiplexing assays.

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For research use only. Not for use in diagnostic procedures.

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AP125TB-C
250 µg
587.00 USD
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AP125TB-M
5 mg
5900.00 USD
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AP125TB-R
25 mg
24100.00 USD
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Detail Information

The Streptavidin coated AlphaPlex™ 545 (Terbium) Acceptor beads are used to capture a wide variety of biotinylated reagents, ranging from small nucleotides to very large proteins. To avoid steric hindrance, it is recommended to use biotin derivatives including an aliphatic spacer composed of at least six carbons. These beads can be used in conjunction with Alpha Donor beads to create no-wash assays for:

  • Protein-protein interactions
  • Protein-DNA interactions
  • Protein-RNA interactions
  • Protein-small molecule interactions
  • Analyte detection
  • Biomarker detection
  • Antibody detection
  • Enzymatic assays
  • Other immunoassays

AlphaPlex Acceptor beads are intended to be used in multiplexing assays, in conjunction with AlphaLISA Acceptor beads and Alpha Donor beads. In a typical Alpha assay, 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 µL reaction volume.

Specifications

Antibody Conjugates Streptavidin
Automation Compatible Yes
Bead Type or Core Bead Type AlphaPlex Terbium (545) Acceptor
Detection Method Alpha
Product Brand Name AlphaPlex
Shipping Condition Blue Ice
Unit Size 5 mg
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Brochure

Guide

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.

PDF 380 KB
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.

PDF 3 MB