LANCE Eu-W8044-DTA Chelate and Eu Standard, 2 mg

LANCE® Eu-W8044-DTA Chelate is optimized for the labeling of proteins and peptides containing at least one primary aliphatic amino group. The labeled compound can be used in LANCE® homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) assays and fluorescence quenching assays (TF-FQA).

• More stable; can be used in assays with concentration of EDTA up to 250 mM (under pH neutral conditions), or when the pH of your assay is low (down to pH 4).
• In LANCE assays, APC (allophycothiocyanin) or ULight-labeled compounds can be used as an acceptor. 
• Dichlorotriazine activated chelates, are suitable for labeling proteins, peptides, nucleic acids and small molecules containing at least one primary aliphatic amino group.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC)]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).