PerkinElmer
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steadylite plus Reporter Gene Assay System, 100 mL

Reporter gene assay with longest-lasting signal for quantitation of firefly luciferase in mammalian cells.

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6066756
10 mL
80.00 USD
 
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6066751
100 mL
606.00 USD
 
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6066759
1000 mL
5100.00 USD
 
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Overview

steadylite plus™ is a homogeneous, high sensitivity luciferase reporter gene assay system for quantitation of firefly luciferase activity in mammalian cells. steadylite plus was designed to provide an extended signal half-life of more than 4 hours, making it the detection system of choice for batch processing systems where a large number of processed plates can wait in stacks before measurement. steadylite plus is suitable for 96-, 384-, or 1536-well microplate formats, and is ideal for:

  • High throughput assays employing a batch processing methodology
  • Any experimental design where detection is delayed, requiring a strong, steady, and long-lived luminescent signal

As with all members of PerkinElmer's Lites suite of luciferase detection reagents, steadylite plus offers superior performance in an easy-to-use format, providing the following benefits:

  • Convenient One-Step Processing: No washes required - simply mix, incubate and read
  • Enhanced Stability: All lites reagents are designed for extended storage at 2-8°C
  • No DTT: Unlike most luciferase detection reagents, lites products do not contain DTT, eliminating toxic hazards and the need for hood work
  • Excellent Z' Values: All lites products offer robust, sensitive performance, with high signal-to-background ratios and excellent Z' values

Specifications

Automation Compatible Yes
Detection Method Luminescence
Experimental Type In vitro
Product Brand Name steadylite plus
Quantity in a Package Amount 1.0 Units
Shipping Condition Ambient
Unit Size 100 mL
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White Paper

Cell-Based Assays: Purposeful Screens for Better Results

Over these last few decades there has been a growing trend in drug discovery to use cellular systems and functional assays, in addition to biochemical assays, for the characterization of new potential therapeutics. The ability to study the interaction between a candidate drug and its target within the context of a whole, intact cell allows for more physiologically relevant data to be obtained. However, such assays are more complex than traditional biochemical assays as such facts as membrane permeability, cellular metabolism, cell variability, additional binding partners, and signal transduction must be considered.

To help you navigate the complexities in designing cell-based assays, we have gathered insights collected over the years and compiled them to provide you with elements to consider when setting up your cell-based assays. After all, any assay, biochemical or cell-based, is only as good as its design.

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