The AlphaLISA® SureFire® Ultra™ Multiplex phospho-SMAD2 (Ser465/467) + Total SMAD2 assay kit is used to measure both the phosphorylation of SMAD2 and total levels of endogenous SMAD2 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure SMAD2 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
For research use only. Not for use in diagnostic procedures.
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The Alpha SureFire® Ultra™ Multiplex p-SMAD (Ser465/467) (Eu) + Total SMAD2 (Tb)assay kit is used to measure both phosphorylated and total levels of endogenous SMAD in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated SMAD2 analysis, and the 545 nm (Tb) signal corresponds to the total SMAD2 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2 agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
In this assay, the Alpha 615 Acceptor bead is directly-conjugated with an antibody to the phosphorylated site on the target protein. The Alpha 545 Acceptor bead is coated with the CaptSure™ agent, which binds the CaptSure tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
|Assay Target Class||Phosphoprotein, Protein|
|Experimental Type||In vitro|
|Product Brand Name||AlphaLISA SureFire Ultra Multiplex|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|
Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.
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