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Alpha SureFire Ultra Multiplex Phospho/Total SMAD2 Assay Kit, 500 Assay Points

The AlphaLISA® SureFire® Ultra™ Multiplex phospho-SMAD2 (Ser465/467) + Total SMAD2 assay kit is used to measure both the phosphorylation of SMAD2 and total levels of endogenous SMAD2 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure SMAD2 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
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MPSU-PTSM2-K500
500 Assay Points
3197.00 USD
 
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MPSU-PTSM2-K10K
10,000 Assay Points
19300.00 USD
 
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MPSU-PTSM2-K50K
50,000 Assay Points
60600.00 USD
 
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MPSU-PTSM2-K-HV
100 Assay Points
946.00 USD
 
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Overview

The Alpha SureFire® Ultra™ Multiplex p-SMAD (Ser465/467) (Eu) + Total SMAD2 (Tb)assay kit is used to measure both phosphorylated and total levels of endogenous SMAD in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated SMAD2 analysis, and the 545 nm (Tb) signal corresponds to the total SMAD2 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2 agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

In this assay, the Alpha 615 Acceptor bead is directly-conjugated with an antibody to the phosphorylated site on the target protein. The Alpha 545 Acceptor bead is coated with the CaptSure™ agent, which binds the CaptSure tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Assay Target SMAD2
Assay Target Class Phosphoprotein, Protein
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name Alpha SureFire Ultra Multiplex
Shipping Condition Blue Ice
Therapeutic Area Oncology, Metabolism
Unit Size 500 Assay Points
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Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

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Drug Discovery Screening Solutions Brochure

Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automated liquid handling, assays and reagents, imaging and detection systems, and informatics.

Working independently or together, with our solutions you can achieve consistent and accurate results. By accelerating the identification and characterization of effective and safe drug candidates, the PerkinElmer portfolio enables you to optimize efficiency in your lab and deliver more actionable, real-world results.

Download the brochure to learn more about how we can partner with you so that you can discover smarter, more effective, data-driven breakthroughs in the critical screening stages of drug discovery and development.

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