The Alpha SureFire® Ultra™ Multiplex phospho-NFκB p65 (Ser536) + Total NFκB p65 assay kit is used to measure both the phosphorylation (on Ser536) and total levels of endogenous NFκB in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure NFκB phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.
true falseFor research use only. Not for use in diagnostic procedures.
The Alpha SureFire Ultra Multiplex p-NFκB + Total NFκB assay kit is used to measure both phosphorylated and total levels of endogenous NFκB in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated NFκB analysis, and the 545 nm (Tb) signal corresponds to the total NFκB analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
| Assay Target | NFκB |
|---|---|
| Assay Target Class | Phosphoprotein, Protein |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Product Brand Name | Alpha SureFire Ultra Multiplex |
| Shipping Condition | Blue Ice |
| Unit Size | 50,000 Assay Points |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater assay throughput and sensitivity has evolved. In response, more robust immunoassays have been developed to address some of the limitations of the standard, colorimetric ELISA.
Find out about the most common limitations of traditional ELISAs and how different ELISA alternative technologies address these limitations.
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OptiPlate-384, White Opaque 384-well Microplate, Case 50
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