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Positive control lysate for Alpha SureFire Ultra Multiplex p-PDGF Receptor β (Tyr751) (Eu)+ Total PDGF Receptor β (Tb) Kit

Positive control lysate for Alpha SureFire Ultra Multiplex p-PDGF Receptor β (Tyr751) (Eu) + Total PDGF Receptor β (Tb) kit

For research use only. Not for use in diagnostic procedures.

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MPSU-PTPDGF-K-L
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NULL OR EMPTY CART

Overview

This positive control lysate is prepared from NIH/3T3 cells, cultured to 80-90% confluence in T175 flasks in 10% FBS containing medium, medium removed and then treated with 50 ng/mL PDGF-BB for 5 minutes and lysed in 5 mL of 1X SureFire Ultra Lysis buffer.

Specifications

Assay Target Class Phosphoprotein, Protein
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra Multiplex
Shipping Condition Blue Ice
Therapeutic Area Oncology, Metabolic
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Application Note

Alpha Terbium SureFire UltraMultiplex: Simultaneous Dual Phosphoprotein Target Analysis

Evaluating pharmaceutical compound efficacy and safety in regulating cell behavior can involve the study of multiple signal transduction pathways and measuring more than one cellular target can provide greater confidence of positive compound hits on the cellular target of interest. Protein phosph ...

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Simultaneous Detection of Total and Phosphorylated Protein in Cellular Kinase Assay Using Alpha SureFire Ultra Multiplex Technology

Cellular kinase signal transduction pathways are involved in the regulation of many important cellular processes such as cell survival, differentiation, and apoptosis. Kinase signaling networks are typically characterized by multiple kinases arranged in cascades containing nodes with feedback loo ...

PDF 1 MB

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Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...

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