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Positive control lysate for Alpha SureFire Ultra Multiplex p-PDGF Receptor β (Tyr751) (Eu)+ Total PDGF Receptor β (Tb) Kit

Positive control lysate for Alpha SureFire Ultra Multiplex p-PDGF Receptor β (Tyr751) (Eu) + Total PDGF Receptor β (Tb) kit

For research use only. Not for use in diagnostic procedures.

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This positive control lysate is prepared from NIH/3T3 cells, cultured to 80-90% confluence in T175 flasks in 10% FBS containing medium, medium removed and then treated with 50 ng/mL PDGF-BB for 5 minutes and lysed in 5 mL of 1X SureFire Ultra Lysis buffer.


Assay Target Class Phosphoprotein, Protein
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra Multiplex
Shipping Condition Blue Ice
Therapeutic Area Oncology, NASH
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Application Note

Alpha Terbium SureFire UltraMultiplex: Simultaneous Dual Phosphoprotein Target Analysis

Evaluating pharmaceutical compound efficacy and safety in regulating cell behavior can involve the study of multiple signal transduction pathways and measuring more than one cellular target can provide greater confidence of positive compound hits on the cellular target of interest. Protein phosphorylation has been identified as a useful readout of cellular activation or inhibition, and these pathways are commonly targeted for therapeutic modulation of disease.

Numerous assay technologies are available to look at protein phosphorylation for drug development, including the AlphaLISA SureFire Ultra which provides a highly sensitive, fully homogenous option for the analysis of signaling pathways. In this application note, we describe the implementation of multiplexing with the AlphaLISA SureFire Ultra Multiplex assay, the only multiplexing platform that is truly homogenous and high throughput.

Simultaneous Detection of Total and Phosphorylated Protein in Cellular Kinase Assay Using Alpha SureFire Ultra Multiplex Technology

Cellular kinase signal transduction pathways are involved in the regulation of many important cellular processes such as cell survival, differentiation, and apoptosis. Kinase signaling networks are typically characterized by multiple kinases arranged in cascades containing nodes with feedback loops and crosstalk between pathways. Numerous assay technologies have been developed for studying kinase signaling pathways, and for screening compound libraries in search of agents to modify receptors or kinase activities. The quality of these assays can be impacted by data variability due to cell seeding inhomogeneity or from compound toxicity. A common method for controlling for variability is to normalize the assay signal to a cellular protein whose level does not change as a function of the treatment.

Alpha SureFire® Ultra Multiplex Phospho and Total assays utilize two types of Alpha Acceptor beads to simultaneously measure two signals from each assay well to easily normalize the assay. Here, we demonstrate the benefit and utility of normalizing assay signal of phosphorylated protein levels to total protein levels in two different cellular models: ERK 1/2 phosphorylation in human melanoma A375 cells and AKT 1/2/3 phosphorylation in mouse myoblast C2C12 cells.



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