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Alpha SureFire® Ultra™ Multiplex phospho/total BTK Assay Kit, 500 Assay Points

The Alpha SureFire® Ultra™ Multiplex phospho-BTK (Tyr223) + Total BTK assay kit is used to measure both the phosphorylation (on Tyr223) and total levels of endogenous BTK in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure BTK phosphorylation by either recombinant or endogenous receptors, and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
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MPSU-PTBTK-K-HV
100 Assay Points
1114.00 USD
 
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MPSU-PTBTK-K500
500 Assay Points
3765.00 USD
 
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MPSU-PTBTK-K10K
10,000 Assay Points
22700.00 USD
 
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MPSU-PTBTK-K50K
50,000 Assay Points
71400.00 USD
 
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Overview

The Alpha SureFire Ultra Multiplex p-BTK + Total BTX assay kit is used to measure both phosphorylated and total levels of endogenous BTK in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated BTX analysis, and the 545 nm (Tb) signal corresponds to the total BTXanalysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Assay Target BTK
Assay Target Class Phosphoprotein, Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA SureFire Ultra Multiplex
Shipping Condition Blue Ice
Unit Size 500 Assay Points
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Application Brief

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Application Note

Alpha Terbium SureFire UltraMultiplex: Simultaneous Dual Phosphoprotein Target Analysis

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Simultaneous Detection of Total and Phosphorylated Protein in Cellular Kinase Assay Using Alpha SureFire Ultra Multiplex Technology

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