The AlphaLISA® SureFire® Ultra™ Multiplex phospho-Akt1/2/3(Ser473) + Total Akt1 assay kit is used to measure both the phosphorylation of Akt1/2/3 and total levels of endogenous Akt1 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt1/2/3 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
true falseFor research use only. Not for use in diagnostic procedures.
The Alpha SureFire Ultra Multiplex p-Akt1/2/3 + Total Akt1 assay kit is used to measure both phosphorylated Akt1/2/3 and total levels of endogenous Akt1 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated Akt1 analysis, and the 545 nm (Tb) signal corresponds to the total Akt1 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
In this assay, the Alpha 615 Acceptor Bead is directly-conjugated with an antibody to the phosphorylated site on the target protein. The Alpha 545 Acceptor Bead is coated with the CaptSureTM agent, which binds the CaptSure tagged anti-total target protein antibody. The Alpha Donor Bead binds the biotinylated anti-total target protein antibody.
| Assay Target | Akt1 |
|---|---|
| Assay Target Class | Protein |
| Assay Technology | Alpha SureFire Ultra Multiplex |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Shipping Condition | Blue Ice |
| Unit Size | 500 assay points |
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OptiPlate-384, White Opaque 384-well Microplate, Case 50
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AlphaPlate-384, Light gray, untreated, Case of 50
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