PerkinElmer
check quantity

Alpha SureFire Ultra Multiplex p-ATK1/2/3(Ser473)/Total AKT1 - 500 Assay Points

The AlphaLISA® SureFire® Ultra Multiplex phospho-Akt1/2/3(Ser473) + Total Akt1 assay kit is used to measure both the phosphorylation of Akt1/2/3 and total levels of endogenous Akt1 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt1/2/3 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only. Not for use in diagnostic procedures.

Part Number
Unit Size
List Price
Your Price
Quantity
MPSU-PTAKT-M500
500 assay points
3065.00 USD
 
more
MPSU-PTAKT-M10K
10,000 assay points
16800.00 USD
 
more
MPSU-PTAKT-M50K
50,000 assay points
52700.00 USD
 
more
MPSU-PTAKT-M-HV
100 assay points
907.00 USD
 
more
Buy Now

Please enter valid quantity

Please log in to add favorites.

NULL OR EMPTY CART

Overview

The Alpha SureFire Ultra Multiplex p-Akt1/2/3 + Total Akt1 assay kit is used to measure both phosphorylated Akt1/2/3 and total levels of endogenous Akt1 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated Akt1 analysis, and the 545 nm (Tb) signal corresponds to the total Akt1 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor Bead is directly-conjugated with an antibody to the phosphorylated site on the target protein. The Alpha 545 Acceptor Bead is coated with the CaptSureTM agent, which binds the CaptSure tagged anti-total target protein antibody. The Alpha Donor Bead binds the biotinylated anti-total target protein antibody.

Specifications

Assay Target Akt1
Assay Target Class Protein
Assay Technology Alpha SureFire Ultra Multiplex
Automation Compatible Yes
Detection Method Alpha
Shipping Condition Blue Ice
Unit Size 500 assay points