Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.
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AequoScreen Double Transfected Cell Line: Human recombinant 5-HT3A receptor in aequorin HEK-293 host cell.
We provide two vials of cryopreserved cells (approximately 2.5 x 106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant channel in a functional assay.
All cell lines are tested for the absence of mycoplasma.
Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
Disclaimer: This product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
|Assay Target Class||Ion channel|
|Assay Target Type||Cell line|
|Assay Validated||Calcium Luminescence|
|Product Brand Name||AequoScreen|
|Quantity in a Package Amount||5.0 Million Cells|
|Receptor Type||5-Hydroxytryptamine (Serotonin)|
|Second Messenger Release||Calcium flux|
|Shipping Condition||Dry Ice|
|Therapeutic Area||Central Nervous System|
|Unit Size||2 vials|
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|Resource Type||File Name||File Format|
|Brochure||Take the Stress Out of Functional Testing - AequoZen and cAMPZen ready-to-use cells||PDF 73 KB|
|Application Note||AequoScreen aequorin-encoding Plasmids for performing aequorin luminescent assay starting from non-aequorin cell lines||PDF 607 KB|
|Application Note||Aequorin bioluminescence assays on MicroBeta2 LumiJET for studying Ca2+-coupled GPCRs and ion channels||PDF 152 KB|
|Poster||New Microplate Luminescence Counter for Aequorin based Ca2+-coupled GPCR Assays||PDF 341 KB|
|Poster||Receptor-Mediated MAPK and Akt Signaling are Maintained in Gamma-Irradiated FroZen Cells||PDF 376 KB|
|Flyer||Aequorin FLIPR||PDF 1 MB|