This product contains living cells. Some of our cAMPZen™ FroZen Cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
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Frozen Cells validated for cAMP testing: Serotonin 5-HT1A receptor, human recombinant, in CHO-K1 host cell. We provide one vial of frozen cells (2.5 million cells/vial). Some of our Frozen cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information. You are authorized to utilize these frozen cell preparations one time only. Any attempt to transfer, re-use, or propagate these cells is expressly unauthorized and a violation of the product terms and conditions of sale.
|Assay Target Class||GPCR|
|Assay Target Type||Cell line|
|Assay Validated||cAMP, Downstream Phosphorylation|
|G-Alpha Natural Receptor||Gi/Go|
|Product Brand Name||cAMPZen|
|Receptor Type||5-Hydroxytryptamine (Serotonin)|
|Second Messenger Release||cAMP, GTP exchange|
|Shipping Condition||Dry Ice|
|Therapeutic Area||Central Nervous System, Cardiovascular, Inflammation, Metabolic, Gastrointestinal|
|Unit Size||2.5 million cells|
Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.
Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute the activea equorin enzyme.
Now you can characterize cellular signaling mechanisms and better understand the complexity of multiple signaling pathways more efficiently. Only the EnSpire® Multimode Plate Reader offers established Corning® Epic® label-free technology in addition to labeled technologies all within one flexible benchtop reader. This exclusive cellular and biochemical label-free platform, in conjunction with labeled technologies, provides a unique orthogonal approach, enabling you to make better decisions faster.
PerkinElmer’s validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. So you can concentrate on results.
Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance