Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
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ROX labeled dideoxyadenosine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations via single base extension.
Fluorescent dideoxynucleotide analogs are 3’-end chain terminators used for DNA sequencing, RFLP mapping, DNA fingerprinting, and point mutation screening analysis.