ROX labeled dideoxyadenosine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations via single base extension.
| Detection Method | Fluorescence |
|---|---|
| Fluorophore | ROX |
| Nucleotide | ddATP |
| Shipping Condition | Ambient |
Fluorescent dideoxynucleotide analogs are 3’-end chain terminators used for DNA sequencing, RFLP mapping, DNA fingerprinting, and point mutation screening analysis.
