Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
true falseROX labeled dideoxyadenosine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations via single base extension.
| buffer | 10 mM Tris-HCL, pH 7.6, 0.1 mM EDTA |
|---|---|
| Concentration | 0.1 mM |
| Detection Method | Fluorescence |
| Fluorophore | ROX |
| Nucleotide | ddATP |
| Shipping Condition | Ambient |
| Unit Size | 2.5 nmol |
Fluorescent dideoxynucleotide analogs are 3’-end chain terminators used for DNA sequencing, RFLP mapping, DNA fingerprinting, and point mutation screening analysis.
