Quasar™ SPP PFP LC columns consists of a pentafluorophenyl (PFP), bonded phase which provides π-π interactions to facilitate alternative selectivity.
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|Inner Diameter||3 mm|
|Particle Size||2.6 µm|
|Product Brand Name||Quasar|
The knowledge about tocochromanol-related compounds, especially tocotrienols, tocodienols,tocomonoenols, and others, is still limited due to several challenges faced in analytical chemistry. These challenges include separation resolution, co-elution, price and absence of standards, and low analyte concentration in plant material. Application of different column stationary phase chemistries can assist in the challenges faced in compound separation.
Superficially porous particle (SPP) columns can also be used to improve separation of these compounds. SPP particles are made of a solid, non-porous core surrounded by a shell of a porous silica material, resulting in a shorter diffusion path in comparison with fully porous based columns. With a shorter diffusion path within the SPP particle itself, coupled with a uniform packed bed and ultra-inert silica surface, reductions in run times can be observed. Such phases benefit from increased efficiency, with separation resembling that of a UHPLC column. They can be used on standard HPLC instrumentation, without concerns regarding high backpressures, which often compromise column longevity.
In this application brief, five different Quasar™ SPP column phases were screened for the separation of four tocopherol and four tocotrienol homologs, with focus on resolving β and γ isomers.
Salbutamol (also known as albuterol) is a bronchodilating agent which is ubiquitously used in the treatment of asthma and chronic obstructive pulmonary disorder (COPD). It is a polar hydrophilic compound (see Figure 1) which can be problematic when analyzing using reverse phase HPLC. Polar compounds can elute very close to the solvent front. It is therefore ideal for an eluting compound to have a capacity factor (k) between 1 and 10. Polar compounds with poor retention can have k values close to zero. Analyses with this level of retention are not reproducible and so it is important to increase the affinity that polar compounds have for the stationary phase. The technical note investigates the impact on retention of salbutamol by changing the chemistry of the stationary phase. When analyzing salbutamol in accordance with the British Pharmacopeia, the monograph stipulates the use of 'end-capped octadecylsilyl silica gel for chromatography (5 µm)'. This definition does not specify the type of end capping giving scope to the evaluation of different stationary phase whilst still adhering to BP requirements. Salbutamol sulfate was analyzed according to the British Pharmacopeia monograph: Salbutamol Pressurised Inhalation, Suspension. This analysis was performed on four Quasar columns: C18, AQ, AQ Plus and SPP C18/PFP in order to obtain the maximum improvement in the retention of salbutamol.