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|Inner Diameter||4.6 mm|
|Particle Size||5.0 µm|
|Product Brand Name||Quasar|
The knowledge about tocochromanol-related compounds, especially tocotrienols, tocodienols,tocomonoenols, and others, is still limited due to several challenges faced in analytical chemistry. These challenges include separation resolution, co-elution, price and absence of standards, and low analyte concentration in plant material. Application of different column stationary phase chemistries can assist in the challenges faced in compound separation.
Superficially porous particle (SPP) columns can also be used to improve separation of these compounds. SPP particles are made of a solid, non-porous core surrounded by a shell of a porous silica material, resulting in a shorter diffusion path in comparison with fully porous based columns. With a shorter diffusion path within the SPP particle itself, coupled with a uniform packed bed and ultra-inert silica surface, reductions in run times can be observed. Such phases benefit from increased efficiency, with separation resembling that of a UHPLC column. They can be used on standard HPLC instrumentation, without concerns regarding high backpressures, which often compromise column longevity.
In this application brief, five different Quasar™ SPP column phases were screened for the separation of four tocopherol and four tocotrienol homologs, with focus on resolving β and γ isomers.
The most commonly used LC reversed-phase alkyl bonded stationary phase is octadecyl carbon chain (C18)-bonded silica, which is denoted as USP classification L1. C18 columns have a broad applicability from pharmaceuticals to food and environmental analyses. However, not all C18 columns are alike. Simply swapping a C18 column from one manufacturer to another can result in differences in retention time, resolution and even selectivity. Differences can arise due to variations in hydrophobicity, silanol activity, packing quality, particle size distribution, and silica purity, to name a few.
This technical note provides details of a comparative study between twenty-four silica-based C18 phases, from a number of manufacturers covering the following areas: