Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.
false falseThis product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
AequoScreen® Double Transfected Cell Line: Human recombinant P2Y11 receptor in aequorin 1321N1 host cell. We provide two vials of cryopreserved cells (approximately 2.5 x 106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant receptor in a functional assay. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
Features:
The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
| Assay Target Class | GPCR |
|---|---|
| Assay Target Type | Cell line |
| Assay Validated | Calcium Luminescence |
| G-Alpha Natural Receptor | Gq/G11 |
| Host Cell | 1321N1 |
| Product Brand Name | AequoScreen |
| Receptor Type | Purinergic P2Y |
| Second Messenger Release | Calcium flux |
| Shipping Condition | Dry Ice |
| Therapeutic Area | Inflammation, Metabolic |
| Unit Size | 5 million cells |
Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.
Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute the activea equorin enzyme.
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