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Purinergic P2Y₁₁ AequoScreen Cell Line

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Stable, recombinant AequoScreen^® cell line expressing aequorin and the Purinergic receptor P2Y11, human recombinant in 1321N1 host cells.

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Part Number ES-293-A

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Cell Line Terms and Conditions must be accepted before orders are placed. Additional agreement needed for commercial use.

Overview
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Overview

PerkinElmer’s AequoScreen^® double transfected cell lines are provided in two vials of frozen cells, each containing ∽2.5 x 10⁶ cells. Detailed product information as well as recommended cell culture conditions and validation assay date are available for each cell line.

The AequoScreen technology is a generic GPCR technology that can be used with G_s, G_i, and G_q-coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

Features:

Fully automatable and miniaturizable to 1536
Enables reduction in reagents and consumable costs
High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
Robust assay (Z′, CV values, solvent resistance)
Reduces the number of false positives from autofluorescent compounds in screening libraries
Option to work with cells in suspension to increase throughput
Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

PerkinElmer’s AequoScreen cell lines require accepted cell line Terms and Conditions prior to order processing. Additional agreement needed for commercial use. Contact us using the Request More Information button above for additional information regarding required documentation.

Specifications

Assay Target Class	GPCR
Assay Target Type	Cell line
Assay Validated	Calcium Luminescence
G-Alpha Natural Receptor	Gq/G11
Host Cell	1321N1
Product Brand Name	AequoScreen
Receptor Type	Purinergic P2Y
Second Messenger Release	Calcium flux
Shipping Condition	Dry Ice
Special Ordering Information	Requires accepted Terms and Conditions. Additional agreement needed for commercial use.
Therapeutic Area	Inflammation, Metabolic
Unit Size	5 million cells

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Application Note

AequoScreen aequorin-encoding Plasmids for performing aequorin luminescent assay starting from non-aequorin cell lines

Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.

PDF 607 KB

Aequorin bioluminescence assays on MicroBeta2 LumiJET for studying Ca2+-coupled GPCRs and ion channels

Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute the activea equorin enzyme.

PDF 152 KB