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ProteinEXact Assay Reagent Kit

The LabChip® GXII Touch Capillary Electrophoresis System utilizes a microfluidic chip to rapidly characterize protein samples from 24-, 96- or 384-well plates. The technology automatically stains distains, electrophoretically separates and analyzes the protein samples. Use the system software to automatically analyze data which is immediately available for review or reporting in virtual gel, electropherogram graph or table summary form.

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Choose ProteinEXact Assay for sizing and quantitation application for proteins ranging in size from 6.5 to 250 kDa.


  • Sizing Range: 6.5 kDa - 250 kDa
  • Sizing Precision RSD (CV): <2%
  • Sizing Resolution: ≤10%
  • Linear Dynamic Range: 10-2000 ng/µL
  • Maximum Total Protein Concentration: 2 mg/mL
  • Sensitivity Limit of Detection (LOD): 0.2 ng/µL
  • Quantitation Reproducibility: <10%
  • Maximum Salt Concentration: 1M NaCl at pH 6.5 to 8.5
  • Chip Primes per Reagent Kit: 10
  • Chip Sample Lifetime: 400
  • Sample Analysis Time: 65 sec


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Application Note

High Throughput Platforms to Characterize a Bispecific Antibody Subjected to Forced Degradation

The new bispecific antibody class (bsAbs) has emerged as one of the fastest-growing next-generation antibody therapies, due to its high specificity and low toxicity. bsAbs are designed to have two specific antigen-binding sites, unlike conventional antibody formats. Rapid and accurate functional evaluation methods and stability characterization are required to ensure product quality, safety, and efficacy.

In this study, we evaluated the stability of a bispecific mouse antibody targeting CD200 and CD47 in mouse, proteins over-expressed in many cancers, under heat stress conditions:

  • High throughput HTRF® with TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) to evaluate the effect of heat stress on the binding activity of bsAbs.
  • High throughput LabChip® ProteinEXact to evaluate the fragmentation of monoclonal antibodies after heat stress, and analyze different forms of bsAbs from cell culture supernatants.



Product Note