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[Methyl-14C] Methylated, Protein Molecular Weight Markers, 1µCi (37kBq)

14C-labeled markers are for use in radiometric applications. The eletrophoretic mobility of methylated molecular weight markers is identical to the unmodified individual protein.

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NEC811001UC
1 µCi
486.00 USD
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5 µCi
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Detail Information

14C-labeled protein molecular weight markers for gel electrophoresis and autoradiography. Gel electophoresis is a widely employed technique for determining the molecular weight of proteins. The mobility of a protein on polyacrylamide gels containing sodium dodecylsulfate (SDS) is primarily a function of the size of the macromolecule. 0.02 µCi of each marker is sufficient for overnight detection by autogradiography.

  • Phosphorylase B: 97,400 Da
  • Albumin (BSA): 69,000 Da
  • Gammaglobulin subunit 1: 55,400 Da
  • Gammaglobulin subunit 2: 30,000 Da
  • Ovalbumin: 46,000 Da
  • Cytochrome C: 12,300 Da

Sample preparation for electrophoresis. Just prior to use:

  1. Make sure the solution is homogeneous. Some precipitation of the SDS is likely to occur upon thawing. It can be brought back to solution by gently warming the solution to 37 °C.
  2. Add 2-mercaptoethanol and SDS to your sample loading buffer (5% by volume) in a snap cap polypropylene tube and place in a boiling water bath for 3-5 minutes.

Specifications

Concentration 0.005 mCi/mL
Detection Method Radiometric
Label Position Specifically Labeled
Radioisotope C-14
Radionuclide 14C
Shipping Condition Dry Ice
Special Ordering Information This is a radioactive product - shipping address must have a license to receive radioactive materials.
Unit Size 1 µCi
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Radiometric Reagents Guide 2010-2011

For over 50 years PerkinElmer has been a leading supplier of radiochemicals, liquid scintillation cocktails, vials and nuclear counting detection instruments. Today is no different. We have always been committed to providing you products for all of your radiometric needs and we are still committed today.

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Guide

Carbon-14 Handling Precautions

This document contains general information designed to provide a basic understanding of radiation safety. While we believe the information to be accurate, regulatory requirements may change and information contained herein is not tailored to individual needs.

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Chromium-51 Handling Precautions

This document contains general information designed to provide a basic understanding of radiation safety. While we believe the information to be accurate, regulatory requirements may change and information contained herein is not tailored to individual needs.

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