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Protein Clear HR Reagent Kit

The LabChip® GXII Touch microfluidics Protein Clear HR assay provides rapid (65 seconds/ sample) high-resolution protein purity analysis for monoclonal antibody (mAb) process development formulation, stability and manufacturing quality control. Enabling the detection and visualization of impurities within a sample, down to 5 ng/µL .

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The Protein Clear HR assay is designed for the high-resolution protein purity assessment of monoclonal antibodies (mAbs) within research and development, enabling the detection and visualization of impurities within a sample, down to 5 ng/µL. With the analytical power and speed of the LabChip® GXII Touch system and IntelliChip assay optimization technology, you can accelerate your protein development workflows with unmatched throughput and reproducibility.

Protein Clear HR and Protein QC Reagent Kits are interchangeable. For use with Protein Clear HR LabChips CLS148695 and CLS148696.

  • Detect and visualize impurity peaks in 65 seconds
  • Innovative IntelliChip technology provides intra- and inter-run reproducibility
  • Limit of Detection (LOD) sensitivity of 5 ng/µL
  • Baseline resolution of proteins as close as 0.2%, determined by mass spectrometry

Kit contents:

  • Dye Solution
  • Sample Buffer
  • Protein Gel Matrix
  • Protein Ladder
  • Lower Marker
  • Wash Buffer
  • VeriMAb Standard (reference standard)

System Requirements:

The Protein Clear HR assay requires a LabChip GXII Touch instrument with V1.6 software or newer and Data Reviewer V5.4 or newer.


Product Brand Name LabChip GXII
Research Areas Drug Development & Manufacturing, Drug Discovery & Manufacturing, Food & Agriculture
Sample Type Protein
Shipping Condition Dry Ice


Protein Clear HR Assay Specifications
Parameter Specification
Sizing Range 14-250 kDa
Maximum Sample Concentration 2000 ng/μL
Sizing Resolution* Resolution > 1.0 for VeriMAb reference standard
Sizing Precision RSD (CV) <2%
Relative Migration time Precision RSD (CV) <2%
Percent Purity Reproducibility <0.5% Main Non Reduced IgG
<5% all other peaks
Sensitivity (LOD) 5 ng/μL (mAb, non-reduced main peak)
Linear Concentration Range 10 – 1000 ng/ μL (mAb, non-reduced main peak)
Linearity (R^2) >0.995
Separation Time per sample 65 seconds
Maximum number of samples per calibration 96
Chip Lifetime 400 samples
Reagent Kit Primes 10
Minimum sample volume 2 μL
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Application Note

Characterization of NISTmAb using Protein Clear HR Assay

Enable high-resolution protein purity assessment of monoclonal antibodies w/ Protein Clear HR-assay

PDF 527 KB

Product Note