AlphaPlex™-545 (Terbium) Acceptor beads conjugated to Protein A, for use in Alpha no-wash multiplexing assays.
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AlphaPlex™-545 (Terbium) Acceptor beads conjugated to Protein A. These beads are used to capture immunoglobulins through their heavy chain (Fc region) allowing optimal orientation and antigen detection. Protein A has higher affinity for rabbit, pig, dog and cat IgG. These beads are used in conjunction with Alpha Donor beads to create no-wash assays for:
AlphaPlex Acceptor beads are intended to be used in multiplexing assays, in conjunction with AlphaLISA Acceptor beads and Alpha Donor beads. In a typical Alpha assay, 250 µg of Acceptor beads is sufficient to run 250-500 wells using a 50 µL reaction volume.
|Antibody Conjugates||Protein A|
|Bead Type or Core Bead Type||AlphaPlex Terbium (545) Acceptor|
|Experimental Type||In vitro|
|Product Brand Name||AlphaPlex|
|Quantity in a Package Amount||1.0 Units|
|Shipping Condition||Blue Ice|
|Unit Size||5 mg|
Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.
This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.
AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.