This product contains living cells. Some AequoZen™ FroZen Cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
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FroZen Cells validated for calcium testing: Prokineticine PKR1. Human Recombinant, in CHO-K1 host cell. We provide one vial of frozen cells (10 million cells/vial). Some of our Frozen cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information. You are authorized to utilize these frozen cell preparations one time only. Any attempt to transfer, re-use, or propagate these cells is expressly unauthorized and a violation of the product terms and conditions of sale.
The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. That means that we will culture the cells and freeze them with an optimized protocol to stop all replication. These cells are then stored in liquid nitrogen or simply in a -80°C freezer, thawed and used directly in a functional, cellular GPCR test with previously validated performance.
|Assay Target Class||GPCR|
|Assay Target Type||Cell line|
|Assay Validated||Calcium Luminescence|
|G-Alpha Coupling Protein||Ga16|
|G-Alpha Natural Receptor||Gq/G11|
|Product Brand Name||AequoZen|
|Second Messenger Release||Calcium flux|
|Shipping Condition||Dry Ice|
|Unit Size||10 million cells|
Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which control its activity.
Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute the activea equorin enzyme.
Now you can characterize cellular signaling mechanisms and better understand the complexity of multiple signaling pathways more efficiently. Only the EnSpire® Multimode Plate Reader offers established Corning® Epic® label-free technology in addition to labeled technologies all within one flexible benchtop reader. This exclusive cellular and biochemical label-free platform, in conjunction with labeled technologies, provides a unique orthogonal approach, enabling you to make better decisions faster.
Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance