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PhenoVue Nile Red Lipid Stain

PhenoVue Nile Red Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets. ​

PhenoVue Nile Red Lipid stain exhibits bright yellow fluorescence and is validated for use in imaging microscopy and high-content screening applications.

PhenoVue Nile Red Lipid stain exhibits:

  • maximum excitation wavelength of 512nm and maximum emission wavelength of 585 when complexed with triglycerides
  • maximum excitation wavelength of 552nm and maximum emission wavelength of 638nm when complexed with phospholipids

For research use only

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Overview

PhenoVue Nile Red Lipid stain is a lipophilic organic molecule that is almost nonfluorescent in water and polar solvents. In lipid-rich environments, Nile Red exhibits enhanced yellow fluorescence, as well as red fluorescence to a lesser extent.

Nile Red is commonly used for localization and quantification of intracellular lipid droplets which are ​involved in lipid synthesis, metabolism and transportation as well as protein storage and degradation or viral replication, all of which are related to pathophysiology, including dyslipidemia, obesity, lipodystrophy, diabetes, fatty liver diseases, or atherosclerosis.

Features

Numbers of Vials Per Unit 2
Quantity or Volume Per Vial 10mg (31.4µmoles)
Form Powder
Storage -20°C
Recommended Working Concentration 200nM (0.008ng/mL)​
Maximum Excitation Wavelength

512 nm - complexed with triglycerides
552 nm - complexed with phospholipids*
(*as shown in SpectraViewer below)

Maximum Emission Wavelength 585 nm - complexed with triglycerides
638 nm - complexed with phospholipids*
(*as shown in SpectraViewer below)
Common Filter Set Cy3.5
Live Cell Staining Yes
Fixed Cell Staining Yes. See Product Information Sheet for more information
Equivalent Number of Microplates 10900-32700​ x 96-well microplates
9090-32700​ x 384-well microplates
17040-40900 x 1536-well microplates

Specifications

Automation Compatible Yes
Detection Method Fluorescence
Product Brand Name PhenoVue
Shipping Condition Ambient
Therapeutic Area Cardiovascular Diseases, Metabolic Disorders
Unit Size 2 vials
Resources, Events & More
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Application Note

Cell Painting for Phenotypic Screening

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.

Download our application note to learn how to:

  • Set up optimal imaging parameters and see the effect of different acquisition modes on compound clustering
  • Easily perform the assay using the dyes provided in the PhenoVue Cell Painting kit
  • Extract and analyze more than 5700 cellular features
  • Visualize multidimensional data using the Signals Screening analysis platform

PDF 1 MB

Brochure

PhenoVue Cellular Imaging Reagents Brochure

This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.

PDF 1 MB

Product Info

PhenoVue Nile Red Lipid Stain Product Information Sheet

This is a product information sheet for PhenoVue Nile Red Lipid Stain.

PDF 827 KB