PhenoVue Nile Red Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets.
PhenoVue Nile Red Lipid stain exhibits bright yellow fluorescence and is validated for use in imaging microscopy and high-content screening applications.
PhenoVue Nile Red Lipid stain exhibits:
For research use only
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PhenoVue Nile Red Lipid stain is a lipophilic organic molecule that is almost nonfluorescent in water and polar solvents. In lipid-rich environments, Nile Red exhibits enhanced yellow fluorescence, as well as red fluorescence to a lesser extent.
Nile Red is commonly used for localization and quantification of intracellular lipid droplets which are involved in lipid synthesis, metabolism and transportation as well as protein storage and degradation or viral replication, all of which are related to pathophysiology, including dyslipidemia, obesity, lipodystrophy, diabetes, fatty liver diseases, or atherosclerosis.
|Numbers of Vials Per Unit||2|
|Quantity or Volume Per Vial||10mg (31.4µmoles)|
|Recommended Working Concentration||200nM (0.008ng/mL)|
|Maximum Excitation Wavelength||
512 nm - complexed with triglycerides
|Maximum Emission Wavelength||585 nm - complexed with triglycerides
638 nm - complexed with phospholipids*
(*as shown in SpectraViewer below)
|Common Filter Set||Cy3.5|
|Live Cell Staining||Yes|
|Fixed Cell Staining||Yes. See Product Information Sheet for more information|
|Equivalent Number of Microplates||10900-32700 x 96-well microplates
9090-32700 x 384-well microplates
17040-40900 x 1536-well microplates
|Product Brand Name||PhenoVue™|
|Therapeutic Area||Cardiovascular Diseases, Metabolic Disorders|
|Unit Size||2 vials|
Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.
However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.
Download our application note to learn how to:
This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
This is a product information sheet for PhenoVue Nile Red Lipid Stain.