PhenoVue Hoechst 33342 nuclear stain is a cell-permeable organic molecule that binds to DNA.
PhenoVue Hoechst 33342 nuclear stain exhibits bright blue fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of PerkinElmer's portfolio of cellular imaging reagents, the PhenoVue Hoechst 33342 nuclear stain has a maximum excitation wavelength of 357 nm and a maximum emission wavelength of 540 nm.
For research use only
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PhenoVue Hoechst 33342 Nuclear stain (trihydrochloride form) is a cell-permeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of the minor groove of DNA. When excited by ultraviolet light, Hoechst 33342 nuclear stain exhibits a strong fluorescence at 540nm. It is commonly used to stain cells' nuclear compartment.
PhenoVue Hoechst 33342 Nuclear stain can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
|Numbers of Vials Per Unit||1|
|Quantity or Volume Per Vial||10mg (10 mg/mL - 16.23mM)|
|Recommended Working Concentration||2µg/mL (3.24µM)|
|Maximum Excitation Wavelength||357 nm|
|Maximum Emission Wavelength||540 nm|
|Common filter set||DAPI|
|Live Cell Staining||Yes|
|Fixed Cell Staining||Yes. See Product Information Sheet for more information|
|Equivalent Number of Microplates||170-520 x 96-well microplates
140-520 x 384-well microplates
270-800 x 1536-well microplates
|Product Brand Name||PhenoVue™|
|Unit Size||1 vial|
Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.
However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.
Download our application note to learn how to:
This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
This is a product information sheet for PhenoVue Hoechst 33342 Nuclear Stain.