PerkinElmer
check quantity

PhenoVue Hoechst 33342 Nuclear Stain 100mg

 

PhenoVue Hoechst 33342 nuclear stain is a cell-permeable organic molecule that binds to DNA.

PhenoVue Hoechst 33342 nuclear stain exhibits bright blue fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of PerkinElmer's portfolio of cellular imaging reagents, the PhenoVue Hoechst 33342 nuclear stain has a maximum excitation wavelength of 357 nm and a maximum emission wavelength of 540 nm.

For research use only

Part Number
Unit Size
List Price
Your Price
Quantity
CP71
10mg
50.00 USD
 
more
CP72
100mg
97.00 USD
 
more
Buy Now

Please enter valid quantity

Please log in to add favorites.

NULL OR EMPTY CART

Overview

PhenoVue Hoechst 33342 Nuclear stain (trihydrochloride form) is a cell-permeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of the minor groove of DNA. When excited by ultraviolet light, Hoechst 33342 nuclear stain exhibits a strong fluorescence at 540nm. It is commonly used to stain cells' nuclear compartment.

 

PhenoVue Hoechst 33342 Nuclear stain can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​

Features

Numbers of Vials Per Unit 1
Quantity or Volume Per Vial 100mg​ (10 mg/mL - 16.23mM)​​
Form Liquid​
Storage -20°C
Recommended Working Concentration 2µg/mL (3.24µM)​
Maximum Excitation Wavelength 357 nm
Maximum Emission Wavelength 540 nm
Common filter set DAPI
Equivalent Number of Microplates 170-520 x 96-well microplates
140-520 x 384-well microplates
270-800​ x 1536-well microplates

Specifications

Automation Compatible Yes
Detection Method Fluorescence
Product Brand Name PhenoVue
Shipping Condition Ambient
Unit Size 100mg
Resources, Events & More
  • All

Application Note

Cell Painting for Phenotypic Screening

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.

Download our application note to learn how to:

  • Set up optimal imaging parameters and see the effect of different acquisition modes on compound clustering
  • Easily perform the assay using the dyes provided in the PhenoVue Cell Painting kit
  • Extract and analyze more than 5700 cellular features
  • Visualize multidimensional data using the Signals Screening analysis platform

PDF 1 MB

Brochure

PhenoVue Cellular Imaging Reagents Brochure

This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.

PDF 3 MB

Product Info

PhenoVue Hoechst 33342 Nuclear Stain Product Information Sheet

This is a product information sheet for PhenoVue Hoechst 33342 Nuclear Stain.

PDF 1 MB