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PhenoVue Fluor 647 - WGA

PhenoVue Fluor 647 - WGA is a fluorescent lectin which displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes. It can be used for cellular membrane staining, particularly the Golgi apparatus.

PhenoVue Fluor 647 - WGA exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of PerkinElmer's portfolio of cellular imaging reagents, PhenoVue Fluor 647 - WGA has a maximum excitation wavelength of 650 nm and a maximum emission wavelength of 670 nm.

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Overview

WGA is a plant lectin that binds different carbohydrate motifs and displays high affinity for sialic acid and N-acetylglucosamine residues of glycoproteins and glycolipids present at the cellular plasma membranes.

Fluorescent WGA derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly Golgi apparatus which is glycoprotein-enriched. In addition, fluorescent WGA derivatives are also routinely used for the staining of skeletal muscle cells and tissues, as well as in cardiac fibrosis.

PhenoVue Fluor 647 - WGA can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Features

Numbers of Vials Per Unit 5
Quantity or Volume Per Unit 1mg (29.2nmoles)​
Form Lyophilized​
Storage 2 - 8°C​
Recommended Working Concentration 5 µg/mL (146nM )
Maximum Excitation Wavelength 650nm
Maximum Emission Wavelength 670nm
Common Filter Set Cy5
Live Cell Staining Yes
Fixed Cell Staining Yes. See Product Information Sheet for more information
Equivalent Number of Microplates 36 - 100 x 96-well microplates
30 - 100​ x 384-well microplates
55 - 160​ x 1536-well microplates

Specifications

Detection Method Fluorescence
Fluorophore PhenoVue Fluor 647
Product Brand Name PhenoVue
Shipping Condition Ambient
Unit Size 5 vials
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Product Info

PhenoVue Fluor - WGA Conjugates Product Information Sheet

PhenoVue Fluor - WGA Conjugates Product Information Sheet

PDF 1 MB