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PhenoVue Fluor 647 - Phalloidin

PhenoVue Fluor 647 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 647 conjugated Phalloidin exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of PerkinElmer's portfolio of cellular imaging reagents, PhenoVue 647 - Phalloidin has a maximum excitation wavelength of 650 nm and a maximum emission wavelength of 670 nm.

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Overview

Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.

Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.

PhenoVue Fluor 647 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Features

Numbers of Vials Per Unit 1
Quantity or Volume Per Unit 300 units (10nmoles; 13µg)
Form Dessicated
Storage -20°C
Recommended Working Concentration 165nM (0.21µg/mL)
Maximum Excitation Wavelength 650nm
Maximum Emission Wavelength 670nm
Common Filter Set Cy5
Live Cell Staining No
Fixed Cell Staining Yes
Equivalent Number of Microplates 2 - 6 x 96-well microplates
1.5 - 6 x 384-well microplates
3 - 10 x 1536-well microplates

Specifications

Detection Method Fluorescence
Fluorophore PhenoVue Fluor 647
Product Brand Name PhenoVue
Shipping Condition Dry Ice
Unit Size 1 vial
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PhenoVue Fluor - Phalloidin Conjugates Product Information Sheet

This is a product information sheet for PhenoVue Fluor - Phalloidin Conjugates

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