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PhenoVue Fluor 594 - Phalloidin

PhenoVue Fluor 594 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 594 conjugated Phalloidin exhibits bright red fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of PerkinElmer's portfolio of cellular imaging reagents, PhenoVue 594 - Phalloidin has a maximum excitation wavelength of 590 nm and a maximum emission wavelength of 617 nm.

For research use only

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Overview

Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.

Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of particular interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.

PhenoVue Fluor 594 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​

Features

Numbers of Vials Per Unit 1
Quantity or Volume Per Vial 300 units (10nmoles; 13µg)
Form Dessicated
Storage -20°C
Recommended Working Concentration 165nM (0.21µg/mL)
Maximum Excitation Wavelength 590 nm
Maximum Emission Wavelength 617 nm
Common Filter Set FITC
Live Cell Staining No
Fixed Cell Staining Yes
Equivalent Number of Microplates 2-6 x 96-well microplates
1.5-6 x 384-well microplates
3-10 x 1536-well microplates

  

Specifications

Automation Compatible Yes
Detection Method Fluorescence
Fluorophore PhenoVue Fluor 594
Product Brand Name PhenoVue
Shipping Condition Ambient
Therapeutic Area Neurology, Cardiovascular Diseases, Fibrosis, Oncology
Unit Size 1 vial
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Application Note

Cell Painting for Phenotypic Screening

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.

Download our application note to learn how to:

  • Set up optimal imaging parameters and see the effect of different acquisition modes on compound clustering
  • Easily perform the assay using the dyes provided in the PhenoVue Cell Painting kit
  • Extract and analyze more than 5700 cellular features
  • Visualize multidimensional data using the Signals Screening analysis platform

PDF 1 MB

Brochure

PhenoVue Cellular Imaging Reagents Brochure

This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.

PDF 1 MB

Product Info

PhenoVue Fluor - Phalloidin Conjugates Product Information Sheet

This is a product information sheet for PhenoVue Fluor - Phalloidin Conjugates

PDF 1 MB