PhenoVue Fluor 568 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.
PhenoVue Fluor 568 conjugated Phalloidin exhibits bright red orange fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of PerkinElmer's portfolio of cellular imaging reagents, PhenoVue 568 - Phalloidin has a maximum excitation wavelength of 578 nm and a maximum emission wavelength of 603 nm.
true falseFor research use only
Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.
Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.
PhenoVue Fluor 568 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
| Numbers of Vials Per Unit | 1 |
|---|---|
| Quantity or Volume Per Vial | 300 units (10nmoles; 13µg) |
| Form | Dessicated |
| Storage | -20°C |
| Recommended Working Concentration | 165nM (0.21µg/mL) |
| Maximum Excitation Wavelength | 578 nm |
| Maximum Emission Wavelength | 603 nm |
| Common Filter Set | FITC |
| Live Cell Staining | No |
| Fixed Cell Staining | Yes |
| Equivalent Number of Microplates | 2-6 x 96-well microplates 1.5-6 x 384-well microplates 3-10 x 1536-well microplates |
| Automation Compatible | Yes |
|---|---|
| Detection Method | Fluorescence |
| Fluorophore | PhenoVue™ Fluor 568 |
| Product Brand Name | PhenoVue™ |
| Shipping Condition | Ambient |
| Therapeutic Area | Neurology, Cardiovascular Diseases, Fibrosis, Oncology |
| Unit Size | 1 vial |
Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.
However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.
Download our application note to learn how to:
This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
This is a product information sheet for PhenoVue Fluor - Phalloidin Conjugates
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