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PhenoVue Fluor 568 - Phalloidin

HeLa cells stained with PhenoVue Fluor 568 - Phalloidin

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PhenoVue Fluor 568 - Phalloidin is a fluorescent dye conjugated bicyclic peptide which binds specifically to F-actin and stains the actin cytoskeleton.

PhenoVue Fluor 568 conjugated Phalloidin exhibits bright red orange fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of PerkinElmer's portfolio of cellular imaging reagents, PhenoVue 568 - Phalloidin has a maximum excitation wavelength of 578 nm and a maximum emission wavelength of 603 nm.

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For research use only

CP25681

430.00 USD

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Overview

Phalloidin is a toxin isolated from the fungus Amanita phalloides. It is a bicyclic peptide that specifically binds and stabilizes actin filaments.

Fluorescent Phalloidin derivatives have been extensively used for monitoring actin expression and dynamics of the cytoskeleton network. The study of actin cytoskeleton regulation is of interest in the context of several pathophysiological processes such as cancer and neurodegenerative diseases as well as fibrosis and vascular diseases.

PhenoVue Fluor 568 - Phalloidin can be used to vizualise actin filaments in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Features

Numbers of Vials Per Unit	1
Quantity or Volume Per Vial	300 units (10nmoles; 13µg)
Form	Dessicated
Storage	-20°C
Recommended Working Concentration	165nM (0.21µg/mL)
Maximum Excitation Wavelength	578 nm
Maximum Emission Wavelength	603 nm
Common Filter Set	FITC
Equivalent Number of Microplates	2-6 x 96-well microplates 1.5-6 x 384-well microplates 3-10 x 1536-well microplates

Specifications

Automation Compatible	Yes
Detection Method	Fluorescence
Fluorophore	PhenoVue^™ Fluor 568
Product Brand Name	PhenoVue^™
Shipping Condition	Ambient
Therapeutic Area	Neurology, Cardiovascular Diseases, Fibrosis, Oncology
Unit Size	1 vial

Resources, Events & More

Application Note

Cell Painting for Phenotypic Screening

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.

Download our application note to learn how to:

Set up optimal imaging parameters and see the effect of different acquisition modes on compound clustering
Easily perform the assay using the dyes provided in the PhenoVue^™ Cell Painting kit
Extract and analyze more than 5700 cellular features
Visualize multidimensional data using the Signals^™ Screening analysis platform

PDF 1 MB

Brochure

PhenoVue Cellular Imaging Reagents Brochure

This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.

PDF 3 MB