Cross-adsorbed goat anti-mouse IgG (H+L) antibody conjugated with PhenoVue™ Fluor 555.
PhenoVue Fluor 555 secondary antibody conjuagtes exhibit bright green fluorescence and are validated for use in high-content screening applications.
Part of PerkinElmer’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 555 goat anti-mouse antibody, cross-adsorbed, has a maximum excitation wavelength of 555nm and a maximum emission wavelength of 570nm.
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PhenoVue Fluor 555 - goat anti-mouse IgG (H+L) antibody, cross-adsorbed, is affinity purified and recognizes IgG1, IgG2a, IgG2b and IgG3 isotypes.
It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
PhenoVue Fluor 555 - goat anti-mouse IgG (H+L) antibody, cross-adsorbed, may display cross-reactivity with other species such as rat or hamster. For improved specificity and lower background, or in multiplexed applications, consider using highly cross-adsorbed PhenoVue Fluor conjugated secondary antibodies as an alternative.
|Numbers of Vials Per Unit||1|
|Quantity or Volume Per Unit||1 mg|
|Recommended Working Concentration||1.5 µg/mL(10nM)|
|Maximum Excitation Wavelength||555nm|
|Maximum Emission Wavelength||570nm|
|Common Filter Set||Cy3|
|Live Cell Staining||No|
|Fixed Cell Staining||Yes|
|Equivalent Number of Microplates||25-70 x 96-well microplates
20-70 x 384-well microplates
35-90 x 1536-well microplates
|Fluorophore||PhenoVue™ Fluor 555|
|Product Brand Name||PhenoVue™|
|Unit Size||1 vial|