Cross-adsorbed donkey anti-rabbit IgG (H+L) antibody conjugated with PhenoVue™ Fluor 555.
PhenoVue Fluor 555 secondary antibody conjugates exhibit bright green fluorescence and are validated for use in high-content screening applications.
Part of PerkinElmer’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 555 donkey anti-rabbit antibody, cross-adsorbed, has a maximum excitation wavelength of 555nm and a maximum emission wavelength of 570nm.
true falsePhenoVue Fluor 555 - donkey anti-rabbit IgG (H+L) antibody, cross-adsorbed, is affinity purified and recognizes rabbit IgG. It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
PhenoVue Fluor 555 - donkey anti-rabbit IgG (H+L) antibody, cross-adsorbed, may display cross-reactivity with other species such as human or Guinea pig. For improved specificity and lower background, or in multiplexed applications, consider using highly cross-adsorbed PhenoVue Fluor conjugated secondary antibodies as an alternative.
| Numbers of Vials Per Unit | 1 |
|---|---|
| Quantity or Volume Per Unit | 1 mg |
| Form | Lyophilized |
| Storage | 2-8°C |
| Recommended Working Concentration | 1.5 µg/mL (10nM) |
| Maximum Excitation Wavelength | 555 nm |
| Maximum Emission Wavelength | 570 nm |
| Common Filter Set | Cy3 |
| Live Cell Staining | No |
| Fixed Cell Staining | Yes |
| Equivalent Number of Microplates |
25-70 x 96-well microplates |
| Automation Compatible | Yes |
|---|---|
| Detection Method | Fluorescence |
| Fluorophore | PhenoVue Fluor 555 |
| Product Brand Name | PhenoVue™ |
| Target Species | Rabbit |
| Unit Size | 1 vial |
Donkey anti-rabbit IgG (H+L) antibodies are conjugated with our bright PhenoVue Fluor dyes.
PhenoVue Fluor 555 - Donkey Anti-Rabbit Antibody Cross-Adsorbed
Detection of EGFR expressed in A431 cells by Indirect immunofluorescence using PhenoVue Fluor 555 - Donkey Anti-Rabbit Antibody Cross-Adsorbed
A431 cells stained with PhenoVue Fluor 555 - Donkey Anti-Rabbit Antibody Cross-Adsorbed
PhenoVue Fluor 555 - Donkey Anti-Rabbit Antibody Cross-Adsorbed
