PhenoVue Fluor 555 - Concanavalin A is a fluorescent lectin which displays high affinity for glycoproteins and glycolipids present at the cellular membranes. It can be used for cellular membrane staining, particularly the endoplasmic reticulum. PhenoVue Fluor 555 - Concanavalin A exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications. Part of PerkinElmer's portfolio of cellular imaging reagents, PhenoVue Fluor 555 - Concanavalin A has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.
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Concanavalin A is a plant homotetrameric lectin known to activate the immune system or induce apoptosis and autophagy. Concanavalin A displays high affinity for α-mannopyranosyl and α-glucopyranosyl residues of glycoproteins and glycolipids present at the cellular membranes. Fluorescent Concanavalin A derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly the endoplasmic reticulum. PhenoVue Fluor 555 - Concanavalin A can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
|Numbers of Vials Per Unit||5|
|Quantity or Volume Per Unit||1mg (9.62nmoles)|
|Storage||2 - 8°C|
|Recommended Working Concentration||100 µg/mL|
|Maximum Excitation Wavelength||555 nm|
|Maximum Emission Wavelength||570 nm|
|Common Filter Set||Cy3|
|Live Cell Staining||Yes|
|Fixed Cell Staining||Yes. See Product Information Sheet for more information|
|Equivalent Number of Microplates||1 - 5 x 96-well microplates
1 - 5 x 384-well microplates
3 - 8 x 1536-well microplates
|Fluorophore||PhenoVue Fluor 555|
|Product Brand Name||PhenoVue|
|Unit Size||5 vials|
Fluorescent Concanavalin A conjugates represent a method of choice for labelling the cellular membranes of mammalian cells, particularly the endoplasmic reticulum which is glycoprotein-enriched.