Cross-adsorbed goat anti-mouse IgG (H+L) antibody conjugated with PhenoVue™ Fluor 488.
PhenoVue Fluor 488 secondary antibody conjuagtes exhibit bright green fluorescence and are validated for use in high-content screening applications.
Part of PerkinElmer’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 488 goat anti-mouse antibody, cross-adsorbed, has a maximum excitation wavelength of 495nm and a maximum emission wavelength of 520nm.
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PhenoVue Fluor 488 - goat anti-mouse IgG (H+L) antibody, cross-adsorbed, is affinity purified and recognizes IgG1, IgG2a, IgG2b and IgG3 isotypes.
It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
PhenoVue Fluor 488 - goat anti-mouse IgG (H+L) antibody, cross-adsorbed, may display cross-reactivity with other species such as rat or hamster. For improved specificity and lower background, or in multiplexed applications, consider using highly cross-adsorbed PhenoVue Fluor conjugated secondary antibodies as an alternative.
| Numbers of Vials Per Unit | 1 |
|---|---|
| Quantity or Volume Per Unit | 1 mg |
| Form | Lyophilized |
| Storage | 2-8°C |
| Recommended Working Concentration | 1.5 µg/mL (10nM) |
| Maximum Excitation Wavelength | 495 nm |
| Maximum Emission Wavelength | 520 nm |
| Common Filter Set | FITC |
| Live Cell Staining | No |
| Fixed Cell Staining | Yes |
| Equivalent Number of Microplates | 25-70 x 96-well microplates 20-70 x 384-well microplates 35-90 x 1536-well microplates |
| Automation Compatible | Yes |
|---|---|
| Detection Method | Fluorescence |
| Fluorophore | PhenoVue Fluor 488 |
| Product Brand Name | PhenoVue™ |
| Shipping Condition | Ambient |
| Target Species | Mouse |
| Unit Size | 1 vial |
This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
This is a product information sheet for PhenoVue Fluor - Goat Anti-Mouse Secondary Antibodies.
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