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PhenoVue Fluor 488 - Donkey Anti-Rabbit Antibody Cross-Adsorbed

Cross-adsorbed donkey anti-rabbit IgG (H+L) antibody conjugated with PhenoVue Fluor 488.

PhenoVue Fluor 488 secondary antibody conjugates exhibit bright green fluorescence and are validated for use in high-content screening applications.

Part of PerkinElmer’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 488 donkey anti-rabbit antibody, cross-adsorbed, has a maximum excitation wavelength of 495nm and a maximum emission wavelength of 520nm.

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PhenoVue Fluor 488 - donkey anti-rabbit IgG (H+L) antibody, cross-adsorbed, is affinity purified and recognizes rabbit IgG. It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

PhenoVue Fluor 488 - donkey anti-rabbit IgG (H+L) antibody, cross-adsorbed, may display cross-reactivity with other species such as human or Guinea pig. For improved specificity and lower background, or in multiplexed applications, consider using highly cross-adsorbed PhenoVue Fluor conjugated secondary antibodies as an alternative.


Numbers of Vials Per Unit 1
Quantity or Volume Per Unit 1 mg
Form Lyophilized
Storage 2-8°C
Recommended Working Concentration 1.5 µg/mL (10nM)
Maximum Excitation Wavelength 495 nm
Maximum Emission Wavelength 520 nm
Common Filter Set FITC
Live Cell Staining No
Fixed Cell Staining Yes
Equivalent Number of Microplates

25-70 x 96-well microplates
20-70 x 384-well microplates
35-90 x 1536-well microplates



Color Green
Detection Method Fluorescence
Filter FITC
Fluorophore PhenoVue Fluor 488
Host Donkey
Product Compatibility Fixed samples only
Product Brand Name PhenoVue
Target Species Rabbit
Type Individual Reagent
Unit Size 1 vial
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Product Info

PhenoVue Fluor - Donkey Anti-Rabbit Secondary Antibodies Product Information Sheet

Donkey anti-rabbit IgG (H+L) antibodies are conjugated with our bright PhenoVue Fluor dyes.