Cross-adsorbed donkey anti-mouse IgG (H+L) antibody conjugated with PhenoVue™ Fluor 488.
PhenoVue Fluor 488 secondary antibody conjugates exhibit bright green fluorescence and are validated for use in high-content screening applications.
Part of PerkinElmer’s portfolio of cellular imaging reagents, the PhenoVue Fluor - 488 donkey anti-rabbit antibody, cross-adsorbed, has a maximum excitation wavelength of 495nm and a maximum emission wavelength of 520nm.
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PhenoVue Fluor 488 - donkey anti-mouse IgG (H+L) antibody, cross-adsorbed, is affinity purified and recognizes IgG1, IgG2a, IgG2b and IgG3 isotypes.
It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
PhenoVue Fluor 488 - donkey anti-mouse IgG (H+L) antibody, cross-adsorbed, may display cross-reactivity with other species such as rat or hamster. For improved specificity and lower background, or in multiplexed applications, consider using highly cross-adsorbed PhenoVue Fluor conjugated secondary antibodies as an alternative.
|Numbers of Vials Per Unit||1|
|Quantity or Volume Per Unit||1 mg|
|Recommended Working Concentration||1.5 µg/mL (10nM)|
|Maximum Excitation Wavelength||495 nm|
|Maximum Emission Wavelength||520 nm|
|Common Filter Set||FITC|
|Live Cell Staining||No|
|Fixed Cell Staining||Yes|
|Equivalent Number of Microplates||25-70 x 96-well microplates
20-70 x 384-well microplates
35-90 x 1536-well microplates
|Fluorophore||PhenoVue™ Fluor 488|
|Product Compatibility||Fixed samples only|
|Product Brand Name||PhenoVue™|
|Unit Size||1 vial|
Donkey anti-mouse IgG (H+L) antibodies are conjugated with our bright PhenoVue Fluor dyes.