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PhenoVue Fluor 488 - Concanavalin A

PhenoVue Fluor 488 - Concanavalin A is a fluorescent lectin which displays high affinity for glycoproteins and glycolipids present at the cellular membranes. It can be used for cellular membrane staining, particularly the endoplasmic reticulum.

PhenoVue Fluor 488 - Concanavalin A exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of PerkinElmer's portfolio of cellular imaging reagents, PhenoVue Fluor 488 - Concanavalin A has a maximum excitation wavelength of 495 nm and a maximum emission wavelength of 520 nm.

For research use only

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Concanavalin A is a plant homotetrameric lectin known to activate the immune system or induce apoptosis and autophagy. Concanavalin A displays high affinity for α-mannopyranosyl and α-glucopyranosyl residues of glycoproteins and glycolipids present at the cellular membranes.

Fluorescent Concanavalin A derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly the endoplasmic reticulum.

PhenoVue Fluor 488 - Concanavalin A can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​


Numbers of Vials Per Unit 5
Quantity or Volume Per Vial 1mg (29.2nmoles)​
Form Lyophilized​
Storage 2-8°C​
Recommended Working Concentration 1mg (9.62nmoles)
Maximum Excitation Wavelength 495 nm
Maximum Emission Wavelength 520 nm
Common filter set FITC​
Live Cell Staining Yes
Fixed Cell Staining Yes. See Product Information Sheet for more information
Equivalent Number of Microplates 1-5 x 96-well microplates
1-5 x 384-well microplates
3-8​ x 1536-well microplates



Automation Compatible Yes
Detection Method Fluorescence
Product Brand Name PhenoVue
Shipping Condition Ambient
Unit Size 5 vials
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Application Note

Cell Painting for Phenotypic Screening

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.

Download our application note to learn how to:

  • Set up optimal imaging parameters and see the effect of different acquisition modes on compound clustering
  • Easily perform the assay using the dyes provided in the PhenoVue Cell Painting kit
  • Extract and analyze more than 5700 cellular features
  • Visualize multidimensional data using the Signals Screening analysis platform



PhenoVue Cellular Imaging Reagents Brochure

This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.


Product Info

PhenoVue Fluor 488 - Concanavalin A Product Information Sheet

This is a product information sheet for PhenoVue Fluor 488 - Concanavalin A.