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PhenoVue Fluor 488 - Concanavalin A

PhenoVue Fluor 488 - Concanavalin A is a fluorescent lectin which displays high affinity for glycoproteins and glycolipids present at the cellular membranes. It can be used for cellular membrane staining, particularly the endoplasmic reticulum.

PhenoVue Fluor 488 - Concanavalin A exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of PerkinElmer's portfolio of cellular imaging reagents, PhenoVue Fluor 488 - Concanavalin A has a maximum excitation wavelength of 495 nm and a maximum emission wavelength of 520 nm.

For research use only

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Overview

Concanavalin A is a plant homotetrameric lectin known to activate the immune system or induce apoptosis and autophagy. Concanavalin A displays high affinity for α-mannopyranosyl and α-glucopyranosyl residues of glycoproteins and glycolipids present at the cellular membranes.

Fluorescent Concanavalin A derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly the endoplasmic reticulum.

PhenoVue Fluor 488 - Concanavalin A can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​

Features

Numbers of Vials Per Unit 5
Quantity or Volume Per Vial 1mg (29.2nmoles)​
Form Lyophilized​
Storage 2-8°C​
Recommended Working Concentration 1mg (9.62nmoles)
Maximum Excitation Wavelength 495 nm
Maximum Emission Wavelength 520 nm
Common filter set FITC​
Equivalent Number of Microplates 1-5 x 96-well microplates
1-5 x 384-well microplates
3-8​ x 1536-well microplates

Specifications

Automation Compatible Yes
Detection Method Fluorescence
Product Brand Name PhenoVue
Shipping Condition Ambient
Unit Size 5 vials
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Application Note

Cell Painting for Phenotypic Screening

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.

Download our application note to learn how to:

  • Set up optimal imaging parameters and see the effect of different acquisition modes on compound clustering
  • Easily perform the assay using the dyes provided in the PhenoVue Cell Painting kit
  • Extract and analyze more than 5700 cellular features
  • Visualize multidimensional data using the Signals Screening analysis platform

PDF 1 MB

Brochure

PhenoVue Cellular Imaging Reagents Brochure

This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.

PDF 3 MB

Product Info

PhenoVue Fluor 488 - Concanavalin A Product Information Sheet

This is a product information sheet for PhenoVue Fluor 488 - Concanavalin A.

PDF 1 MB