PerkinElmer
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PhenoVue DAPI nuclear stain

PhenoVue DAPI nuclear stain is a cell-impermeable organic molecule that binds to DNA and is commonly used to stain cell's nucleus.

Part of PerkinElmer’s portfolio of cellular imaging reagents, the PhenoVue DAPI nuclear stain has a maximum excitation wavelength of 348nm and a maximum emission wavelength of 457nm.

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Overview

PhenoVue DAPI nuclear stain is a cell-impermeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of minor groove of DNA. When excited by ultraviolet light, DAPI exhibits strong fluorescence at 455 nm. It is commonly used to stain cells’ nuclear compartment.

It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​

Features

Numbers of Vials Per Unit 1
Quantity or Volume Per Unit 1 mg (1mg/mL - 2.85mM)
Form Liquid
Storage -20°C
Recommended Working Concentration 1µg/mL (10nM)
Maximum Excitation Wavelength 358 nm
Maximum Emission Wavelength 457 nm
Common Filter Set DAPI
Live Cell Staining usually not, except at high concentrations
Fixed Cell Staining Yes
Equivalent Number of Microplates

350-1040 x 96-well microplates
290-1040 x 384-well microplates 540-1630 x 1536-well microplates

Specifications

Detection Method Fluorescence
Fluorophore DAPI
Product Brand Name PhenoVue™
Shipping Condition Ambient
Unit Size 1 vial
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Product Info

PhenoVue DAPI Nuclear Stain Product Information Sheet

This is the product information sheet for PhenoVue DAPI Nuclear Stain.

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