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PhenoVue Cell Painting JUMP Kit 1 x 384-wells

PhenoVue™ Cell Painting JUMP Kits comprises 6 validated, pre-optimized fluorescent probes, in accordance with the JUMP consortium protocol v3, and associated diluent to streamline the cell painting workflow.

The kits offer excellent batch-to-batch reproducibility, are supplied with a step-by-step protocol, and are available in a range of pack sizes to match your throughput requirements.

PhenoVue Cell Painting JUMP kits are validated for use on high-content screening systems such as the Opera Phenix® Plus system.

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Unit Size
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PING21
For 1 x 384-well microplate
154.00 USD
 
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PING22
For 10 x 384-well microplates
1066.00 USD
 
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PING23
For 100 x 384-well microplates
8000.00 USD
 
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Overview

Cell Painting is a powerful phenotypic high-content screening approach which combines cell and computational biology to unravel cells’ responses when subjected to perturbagens.

Cell are "painted" by labelling different cellular compartments with different fluorescent bioprobes to quantitatively profile multiple phenotypic parameters in order to better understand the effects of chemical compounds, drugs, genes, or other test articles.​

PhenoVue Cell Painting JUMP Kits comprise validated, pre-optimized fluorescent bioprobes, aligned to the JUMP consortium protocol v3, to streamline your workflow, saving time and costs.

PhenoVue Cell Painting JUMP Kits provide:

  • Excellent batch-to-batch reproducibility The cell painting kit reagents are tested extensively (>80 parameters) using state-of-the-art techniques to guarantee high quality e.g. LCMS, HPLC, absorbance spectra, concentration, high-content imaging, degree of labelling (DoL), with strict acceptance criteria to deliver reliable and accurate results.
  • Ease-of-use The reagents in the cell painting kits are supplied in optimized formulations to facilitate assay set-up, together with a detailed step-by-step protocol.
  • Convenient pack sizes The cell painting kits are available in three different sizes, 1 x 384-well plate, 10 x 384-well plates and 100 x 384-well plates to meet your throughput needs and avoid waste.
  • Tried and Trusted used by the Joint Undertaking in Morphological Profiling-Cell Painting (JUMP-CP) consortium in creating the world’s largest, public cell painting dataset. Learn more here: https://ir.perkinelmer.com/news-releases/news-release-details/perkinelmer-supports-jump-cp-consortium-creating-worlds-largest

Kit Components / Quantity

  • PhenoVue Hoechst 33342 Nuclear Stain / 1 vial - 70µg
  • PhenoVue Fluor 488 - Concanavalin A / 1 vial - 1.2mg
  • PhenoVue 512 Nucleic Acid Stain / 1 vial - 100nmol
  • PhenoVue Fluor 555 - WGA / 1 vial - 0.02mg
  • PhenoVue Fluor 568 - Phalloidin / 1 vial - 0.4nmol
  • PhenoVue 641 Mitochondrial Stain / 1 vial - 22µg
  • PhenoVue Dye Diluent A (5X) / 1 vial - 8mL (5X)

Specifications

Detection Method Fluorescence
Product Compatibility Fixed samples only
Product Brand Name PhenoVue™
Quantity in a Package Amount 7.0 Vials
Shipping Condition Dry Ice
Type Kit
Unit Size For 1 x 384-well microplate
Resources, Events & More
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Application Note

Cell Painting for Phenotypic Screening

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - f ...

PDF 1 MB

Article

Cell Painting: Your Questions Answered

With powerful potential for phenotypic drug discovery, cell painting is being rapidly adopted by leading scientists across the globe. However, implementing cell painting assays can present some challenges.

To help you avoid some of the common pitfalls, we’ve collated answers from our expert ...

PDF 470 KB

Brochure

PhenoVue Cellular Imaging Reagents Brochure

This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.

PDF 1 MB

Product Info

PhenoVue Cell Painting JUMP Kit, 1 x 384-wells Product Information Sheet

Details of PhenoVue Cell Painting JUMP Kit, 1 x 384-wells.

PDF 933 KB

Webinars

Cell Painting - A Practical Approach
Webinars

White Paper

Cell Painting - A Cellular Imaging and Machine Learning Approach to Drug Discovery

Phenotypic Drug Discovery Re-Imagined

Cell Painting is a phenotypic screening method and a powerful application of high-content screening technology which combines cell and computational biology to elucidate the behavior of cells under the influence of perturbagens, such as chemical compoun ...

PDF 502 KB
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Image Gallery
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U-2 OS cells stained with PhenoVue Cell Painting JUMP Kit U-2 OS cells stained with PhenoVue Cell Painting JUMP Kit
U-2 OS cells treated or untreated and stained with PhenoVue Cell Painting JUMP Kit U-2 OS cells treated or untreated and stained with PhenoVue Cell Painting JUMP Kit
Purvalanol A treated U-2 OS cells stained with PhenoVue Cell Painting JUMP Kit Purvalanol A treated U-2 OS cells stained with PhenoVue Cell Painting JUMP Kit
Lonafarnib treated U-2 OS cells stained with PhenoVue Cell Painting JUMP Kit Lonafarnib treated U-2 OS cells stained with PhenoVue Cell Painting JUMP Kit
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U-2 OS cells stained with PhenoVue Cell Painting JUMP Kit
U-2 OS cells treated or untreated and stained with PhenoVue Cell Painting JUMP Kit
Purvalanol A treated U-2 OS cells stained with PhenoVue Cell Painting JUMP Kit
Lonafarnib treated U-2 OS cells stained with PhenoVue Cell Painting JUMP Kit