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PhenoVue Cell Painting JUMP Kit 1 x 384-wells

U-2 OS cells stained with PhenoVue Cell Painting JUMP Kit

U-2 OS cells treated or untreated and stained with PhenoVue Cell Painting JUMP Kit

Purvalanol A treated U-2 OS cells stained with PhenoVue Cell Painting JUMP Kit

Lonafarnib treated U-2 OS cells stained with PhenoVue Cell Painting JUMP Kit

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The PhenoVue^™ Cell Painting Kit comprises 6 validated, pre-optimized fluorescent probes, in accordance with the JUMP consortium protocol v3, and associated diluent to streamline the cell painting workflow.

The PhenoVue cell painting kit is validated for use in high-content screening systems such as the Opera Phenix Plus.

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PING21

For 1 x 384-well microplate

130.00 USD

PING22

For 10 x 384-well microplates

899.00 USD

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For 100 x 384-well microplates

7200.00 USD

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Overview
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Overview

Cell Painting is a powerful phenotypic high-content screening approach which combines cell and computational biology to unravel cells’ responses when subjected to perturbagens.

Cell are "painted" by labelling different cellular compartments with different fluorescent bioprobes to quantitatively profile multiple phenotypic parameters in order to better understand the effects of chemical compounds, drugs, genes, or other test articles.

The PhenoVue Cell Painting JUMP Kit comprises validated, pre-optimized fluorescent bioprobes, aligned to the JUMP consortium protocol v3, to streamline your workflow, saving time and costs.

Kit Components / Quantity

PhenoVue Hoechst 33342 Nuclear Stain / 1 vial - 70µg
PhenoVue Fluor 488 - Concanavalin A / 1 vial - 1.2mg
PhenoVue 512 Nucleic Acid Stain / 1 vial - 100nmol
PhenoVue Fluor 555 - WGA / 1 vial - 0.02mg
PhenoVue Fluor 568 - Phalloidin / 1 vial - 0.4nmol
PhenoVue 641 Mitochondrial Stain / 1 vial - 22µg
PhenoVue Dye Diluent A (5X) / 1 vial - 8mL (5X)

Specifications

Automation Compatible	Yes
Detection Method	Fluorescence
Product Brand Name	PhenoVue^™
Quantity in a Package Amount	7.0 Vials
Shipping Condition	Dry Ice
Unit Size	For 1 x 384-well microplate

Resources, Events & More

Application Note

Cell Painting for Phenotypic Screening

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.

Download our application note to learn how to:

Set up optimal imaging parameters and see the effect of different acquisition modes on compound clustering
Easily perform the assay using the dyes provided in the PhenoVue^™ Cell Painting kit
Extract and analyze more than 5700 cellular features
Visualize multidimensional data using the Signals^™ Screening analysis platform

PDF 1 MB

Brochure

PhenoVue Cellular Imaging Reagents Brochure

This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.

PDF 1 MB

Product Info

PhenoVue Cell Painting JUMP Kit, 1 x 384-wells Product Information Sheet

Details of PhenoVue Cell Painting JUMP Kit, 1 x 384-wells.

PDF 897 KB

Webinars

Cell Painting - A Practical Approach

Webinars

White Paper

Cell Painting - A Cellular Imaging and Machine Learning Approach to Drug Discovery

Phenotypic Drug Discovery Re-Imagined

Cell Painting is a phenotypic screening method and a powerful application of high-content screening technology which combines cell and computational biology to elucidate the behavior of cells under the influence of perturbagens, such as chemical compounds, drugs, genes, or other entities.

Download our white paper to learn about the origins of the Cell Painting technique, its potential impact on the drug discovery paradigm, together with practical hints and tips for success.

PDF 342 KB

Tools