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PhenoVue^™ 578 Mitochondrial Stain

HeLa cells stained with PhenoVue 578 Mitochondrial Stain

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PhenoVue 578 Mitochondrial stain is a fluorescent dye which accumulates in heathly mitochondria of live cells.

PhenoVue 578 Mitochondria stain exhibits bright red fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of PerkinElmer's portfolio of cellular imaging reagents, PhenoVue 578 Mitochondrial stain has a maximum excitation wavelength of 578 nm and a maximum emission wavelength of 599 nm, which makes it an alternative to the similar stain MitoTracker^™ Red CMTMRos.

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For research use only

CP3R1

340.00 USD

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Overview
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Overview

PhenoVue 578 Mitochondrial stain is a cationic rosamine based structure. With mitochondrial membrane potential, it accumulates in mitochondria through electrostatic interactions and reacts with thiol moieties forming stable thioether bonds. Therefore PhenoVue 578 Mitochondrial stain is well retained after cell fixation.

Fluorescent mitochondrial stains are commonly used for visualizing and quantifying mitochondria whose dysfunctions, including impaired biogenesis, dynamics or trafficking, are a hallmark of some human diseases, such as neurodegenerative disorders.

PhenoVue 578 Mitochondrial stain can be used to detect mitochondria in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.

Features

Numbers of Vials Per Unit	20
Quantity or Volume Per Vial	50µg (94nmoles)
Form	Dessicated
Storage	-20°C
Recommended Working Concentration	100nM (0.05ng/mL)
Maximum Excitation Wavelength	578 nm
Maximum Emission Wavelength	599 nm
Common Filter Set	Texas-Red
Live Cell Staining	Yes
Fixed Cell Staining	No
Equivalent Number of Microplates	650-1850 x 96-well microplates 550-1950 x 384-well microplates 1000-3050 x 1536-well microplates

Specifications

Automation Compatible	Yes
Detection Method	Fluorescence
Product Brand Name	PhenoVue^™
Shipping Condition	Dry Ice
Therapeutic Area	Neurology, Oncology, Metabolic Disorders
Unit Size	20 vials

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Application Note

Cell Painting for Phenotypic Screening

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.

Download our application note to learn how to:

Set up optimal imaging parameters and see the effect of different acquisition modes on compound clustering
Easily perform the assay using the dyes provided in the PhenoVue^™ Cell Painting kit
Extract and analyze more than 5700 cellular features
Visualize multidimensional data using the Signals^™ Screening analysis platform

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Brochure

PhenoVue Cellular Imaging Reagents Brochure

This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.

PDF 1 MB