PhenoVue 551 Mitochondrial stain is a fluorescent dye which accumulates in heathly mitochondria of live cells.
PhenoVue 551 Mitochondrial stain exhibits bright orange fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of PerkinElmer's portfolio of cellular imaging reagents, PhenoVue 551 Mitochondrial stain has a maximum excitation wavelength of 551 nm and a maximum emission wavelength of 576 nm, which makes it an alternative to the similar stain MitoTracker™ Orange CMTMRos.
For research use only
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PhenoVue 551 Mitochondrial stain is a cationic rosamine based structure. With mitochondrial membrane potential, it accumulates in mitochondria through electrostatic interactions and reacts with thiol moieties forming stable thioether bonds. Therefore PhenoVue 551 Mitochondrial stain is well retained after cell fixation.
Fluorescent mitochondrial stains are commonly used for visualizing and quantifying mitochondria whose dysfunctions, including impaired biogenesis, dynamics or trafficking, are a hallmark of some human diseases, such as neurodegenerative disorders.
PhenoVue 551 Mitochondrial stain can be used to detect mitochondria in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
|Numbers of Vials Per Unit||20|
|Quantity or Volume Per Vial||50µg (117nmoles)|
|Recommended Working Concentration||100nM (0.04ng/mL)|
|Maximum Excitation Wavelength||551 nm|
|Maximum Emission Wavelength||576 nm|
|Common Filter Set||Cy3, RFP|
|Live Cell Staining||Yes|
|Fixed Cell Staining||No|
|Equivalent Number of Microplates||800-2400 x 96-well microplates
650-2400 x 384-well microplates
1250-3800 x 1536-well microplates
|Product Brand Name||PhenoVue™|
|Shipping Condition||Dry Ice|
|Therapeutic Area||Neurology, Oncology, Metabolic Disorders|
|Unit Size||20 vials|
Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.
However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.
Download our application note to learn how to:
This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
This is a product information sheet for PhenoVue Mitochondrial Stains.