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PhenoVue 512 Nucleic Acid Stain

PhenoVue 512 Nucleic acid stain is a cell permeable organic molecule which binds nucleic acids. It displays a higher fluorescence intensity when complexed with RNA compared to DNA and can be used for nucleoli staining.

PhenoVue 512 Nucleic acid stain exhibits bright green or orange fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of PerkinElmer's portfolio of cellular imaging reagents, PhenoVue 512 Nucleic acid stain has a maximum excitation wavelength of 525 nm and a maximum emission wavelength of 590 nm, which makes it an alternative to the similar stain SYTO 14 green fluorescent nucleic acid stain.

For research use only

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Overview

PhenoVue 512 Nucleic acid stain is a cell permeable organic molecule which concentrates into RNA enriched organelles, such as nucleoli found in the nucleus of mammalian cells.

PhenoVue 512 Nucleic acid stain can be used for localization and quantification of nucleoli which are a nuclear sub-compartment that varies in size and number depending on cell type. Nucleoli are involved in the control of many cellular processes that are fundamental to normal cell homeostasis such as synthesis, processing and assembly of ribosomes as well as cell cycle regulation and cellular stress responses. Genetic disorders such as Werner syndrome or fragile X syndrome have been associated with nucleolar proteins. In addition, dysregulations of nucleolus functions have been reported in neurodegenerative diseases, cancer and cardiovascular diseases.

PhenoVue 512 Nucleic acid stain can be used to visualize nucleoli in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications. ​​

Features

Numbers of Vials Per Unit 1
Quantity or Volume Per Vial 5mM ​(1.25 µmoles - 0.60 mg)​
Form Liquid
Storage -20°C
Recommended Working Concentration 3 µM (1.43 µg/mL)​​​
Maximum Excitation Wavelength 512 nm (methanol) / 525 nm (HBSS)
Maximum Emission Wavelength 590 nm (HBSS)
Common Filter Set FITC, Cy3.5​
Equivalent Number of Microplates 15-45 x 96-well microplates
10-45​ x 384-well microplates
20-70​ x 1536-well microplates

Specifications

Automation Compatible Yes
Detection Method Fluorescence
Product Brand Name PhenoVue
Shipping Condition Ambient
Therapeutic Area Neurology, Cardiovascular Diseases, Oncology
Unit Size 1 vials
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Application Note

Cell Painting for Phenotypic Screening

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.

Download our application note to learn how to:

  • Set up optimal imaging parameters and see the effect of different acquisition modes on compound clustering
  • Easily perform the assay using the dyes provided in the PhenoVue Cell Painting kit
  • Extract and analyze more than 5700 cellular features
  • Visualize multidimensional data using the Signals Screening analysis platform

PDF 1 MB

Brochure

PhenoVue Cellular Imaging Reagents Brochure

This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.

PDF 3 MB

Product Info

PhenoVue 512 Nucleic Acid Stain Product Information Sheet

This is a product information sheet for PhenoVue 512 Nucleic Acid Stain.

PDF 1 MB