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Lysosomes are abundant organelles which contain more than sixty acidic enzymes. Lysosomes play essential role in macromolecules and organelles break down through the autophagy-lysosomal system. Defective regulation of lysosomal function has been reported in several diseases such as lysosomal storage disorders and neurodegenerative diseases like Alzheimer’s and Parkinson’s. PhenoVue 503 lysosomal stain is partially protonated at neutral pH, and highly protonated at acidic pH. In living cells, PhenoVue 503 lysosomal stain accumulates in acidic vesicles such as lysosomes, resulting in bright and specific lysosomal fluorescent staining. PhenoVue 503 lysosomal stain can be used to lysosomes in immunofluorescence, as well as high-content analysis and screening applications.
|Numbers of Vials Per Unit||20|
|Quantity or Volume Per Unit||18.1µg (50nmoles)|
|Form||Solution 1mM DMSO|
|Recommended Working Concentration||50 ng/mL|
|Maximum Excitation Wavelength||503 nm|
|Maximum Emission Wavelength||510 nm|
|Common Filter Set||FITC|
|Live Cell Staining||Yes|
|Fixed Cell Staining||No|
|Equivalent Number of Microplates||650 - 2050 x 96-well microplates
550 - 2050 x 384-well microplates
1050 - 3250 x 1536-well microplates
|Fluorophore||PhenoVue™ Fluor 488|
|Product Brand Name||PhenoVue™|
|Shipping Condition||Dry Ice|
|Unit Size||20 vials|
In living cells, PhenoVue lysosomal stains accumulate in acidic vesicles such as lysosomes, resulting in bright and specific lysosomal fluorescent staining.