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PhenoVue 493 Lipid Stain

PhenoVue 493 Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets. ​

PhenoVue 493 Lipid stain exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.

Part of PerkinElmer's portfolio of cellular imaging reagents, PhenoVue 493 Lipid stain has a maximum excitation wavelength of 493 nm and a maximum emission wavelength of 504 nm,which makes it an alternative to the similar stain BODIPY 493/503.

For research use only

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PhenoVue 493 Lipid stain is a lipophilic fluorescent organic molecule that localizes to polar lipids. It rapidly accumulates in lipid droplets and exhibits enhanced green fluorescence in the presence of neutral lipids like triglycerides and cholesterol esters.

PhenoVue 493 Lipid stain is commonly used for localization and quantification of intracellular lipid droplets which are ​involved in lipid synthesis, metabolism and transportation as well as protein storage and degradation or viral replication, all of which are related to pathophysiology, including dyslipidemia, obesity, lipodystrophy, diabetes, fatty liver diseases, or atherosclerosis.


Numbers of Vials Per Unit 5
Quantity or Volume Per Vial 2mg (4mM-1.05g/L)​
Form Liquid
Storage -20°C
Recommended Working Concentration 5µM (1.31mg/L)​​
Maximum Excitation Wavelength 493 nm
Maximum Emission Wavelength 504 nm
Common Filter Set FITC
Live Cell Staining Yes
Fixed Cell Staining Yes. See Product Information Sheet for more information
Equivalent Number of Microplates 270-830​​ x 96-well microplates
230-830​ x 384-well microplates
430-1300​ x 1536-well microplates



Automation Compatible Yes
Detection Method Fluorescence
Product Brand Name PhenoVue
Shipping Condition Dry Ice
Therapeutic Area Cardiovascular Diseases, Metabolic Disorders
Unit Size 2 vials
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Application Note

Cell Painting for Phenotypic Screening

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - from choosing a cell model and labeling reagents, to optimizing instrumentation and making sense of the thousands of features that are extracted during data analysis.

Download our application note to learn how to:

  • Set up optimal imaging parameters and see the effect of different acquisition modes on compound clustering
  • Easily perform the assay using the dyes provided in the PhenoVue Cell Painting kit
  • Extract and analyze more than 5700 cellular features
  • Visualize multidimensional data using the Signals Screening analysis platform



PhenoVue Cellular Imaging Reagents Brochure

This brochure describes PerkinElmer's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.


Product Info

PhenoVue 493 Lipid Stain Product Information Sheet

This is a product information sheet for PhenoVue 493 Lipid Stain Product.