96-well tissue culture treated (TC-treated), poly-D-lysine (PDL) coated ViewPlate™ microplate with glass clear bottom and black well walls for high content analysis applications. Glass bottom microplates from PerkinElmer are not recommended to be used within an elevated temperature environment above 45 °C.
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Our ViewPlate™ microplates are engineered for bottom reading systems and high content imaging. Glass exhibits better optical properties and is perfectly flat but is less-suited for cell culture. Glass therefore usually must be coated with proteins that enhance cell adherence and growth (e.g., poly-D-lysine or collagen). This high quality microplate can be utilized in your cell based assay for superior results.
Poly-lysine is a synthetic positively-charged polymer, existing as two enantiomers: Poly-D-lysine (PDL) and Poly-L-lysine (PLL). Adherence of certain cell types to poly-lysine-coated surfaces is based on the electrostatic interaction of the poly-D-lysine polycation with the negative charges of the cell membrane. Use of poly-lysine coatings on plate surfaces can help mediate the negative charges of the cell membrane and the negative charge of the surface. Both PDL and PLL are commonly used however PDL is not degraded by cellular proteases and is therefore often the preferred choice. As Poly-lysine is a synthetic protein, it does not influence the signaling pathways of the cells and is completely free of any animal contaminants. Almost all cell types will adhere to Poly-lysine coated plate bottoms.
Leveraging years of assay and instrument experience in plate detection, PerkinElmer designs better microplates for better performance that guarantees better results for all PerkinElmer applications.
Glass bottom microplates from PerkinElmer are not recommended to be used within an elevated temperature environment above 45 °C.
Well format: 96-well w/Glass bottom
|Number of Rows||8|
|Number of Columns||12|
|Well volume||300 µL|
|Recommended working volume||50 µL- 200 µL|
|Well diameter (mm)||6|
|Well depth (mm)||11.35|
|A1 to top offset (mm)||11.3|
|A1 to side offset (mm)||14.3|
|Well-to-well spacing (mm)||9|
|Coating Treatment||Sterile, PDL|
|Detection Method||Confocal Imaging, Fluorescence, High Content Imaging|
|Material||Polystyrene, Glass Bottom|
|Product Brand Name||ViewPlate|
|Unit Size||Case of 8|
|Wells Number||96 well plate|
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